General Setup

Setup chunk

Setup reticulate

knitr::opts_chunk$set(fig.width = 8)
knitr::opts_knit$set(root.dir = normalizePath(".."))
knitr::opts_knit$get("root.dir")
[1] "/nas/groups/treutlein/USERS/tomasgomes/projects/liver_regen"

Load libraries

library(reticulate)
knitr::knit_engines$set(python = reticulate::eng_python)
py_available(initialize = FALSE)
[1] TRUE
use_python(Sys.which("python"))
py_config()
python:         /home/tpires/bin/miniconda3/bin/python
libpython:      /home/tpires/bin/miniconda3/lib/libpython3.8.so
pythonhome:     /home/tpires/bin/miniconda3:/home/tpires/bin/miniconda3
version:        3.8.3 (default, May 19 2020, 18:47:26)  [GCC 7.3.0]
numpy:          /home/tpires/bin/miniconda3/lib/python3.8/site-packages/numpy
numpy_version:  1.19.0

NOTE: Python version was forced by RETICULATE_PYTHON

Functions

getDPT = function(x){
  return(destiny:::dpt_for_branch(destiny::branch_divide(x, integer(0L)), 
                                  min(destiny::branch_divide(x, integer(0L))@branch[, 1], 
                                      na.rm = TRUE)))
}
CVgam = function (formula, data, nfold = 10, debug.level = 0, method = "glm.fit", 
    printit = TRUE, cvparts = NULL, seed = 29){
  # modified from the `gamclass` package to use gam::gam
    if (is.null(cvparts)) {
        set.seed(seed)
        cvparts <- sample(1:nfold, nrow(data), replace = TRUE)
    }
    folds <- unique(cvparts)
    khat <- hat <- numeric(nrow(data))
    scale.gam <- summary(gam::gam(formula, data = data, method = method,
                                  family = mgcv::betar(link = "logit", eps = 0.00001)))$scale
    for (i in folds) {
        trainrows <- cvparts != i
        testrows <- cvparts == i
        elev.gam <- gam::gam(formula, data = data[trainrows, ], 
                             method = method, 
                             family = mgcv::betar(link = "logit", eps = 0.00001))
        hat[testrows] <- predict(elev.gam, newdata = data[testrows,], select = T, type = "response")
        res <- residuals(elev.gam)
    }
    y <- eval(formula[[2]], envir = as.data.frame(data))
    res <- y - hat
    cvscale <- sum(res^2)/length(res)
    prntvec <- c(GAMscale = scale.gam, `CV-mse-GAM ` = cvscale)
    if (printit) 
        print(round(prntvec, 4))
    invisible(list(fitted = hat, resid = res, cvscale = cvscale, 
        scale.gam = scale.gam))
}
fittingFunc = function(g){
  t = sub_dat$zonation_pt
  gene_fit_p = c()
  gene_fit_vals = data.frame(row.names = 1:100)
  z = sub_dat@assays$SCT@data[g,]
    
  d = data.frame(z=z, t=t)
  tmp = suppressMessages(gam(z ~ ns(t, df = 3), data=d))
  
  # bins for model fitting
  bb = seq(min(t), max(t), length.out = 100)
  gene_fit_vals[,g] = suppressMessages(predict(tmp,
                                               newdata = data.frame(t = bb)))
  p = summary(tmp)$parametric.anova$`Pr(>F)`[1]
  gene_fit_p = c(gene_fit_p, p)
  names(gene_fit_p)[length(gene_fit_p)] = g
  
  return(list("fits" = gene_fit_vals, "pval" = gene_fit_p))
}
minmax_scale = function(x){
  return((x-min(x))/(max(x)-min(x)))
}
getTopTerms = function(godf, topt = 100, ncl = 5, nt = 2){
  topt = if(nrow(godf)<topt) nrow(godf) else topt
  if(nrow(godf)<ncl) return(godf)
  df = godf[1:topt,]
  genes = sapply(df$geneID, function(x) strsplit(x, "/"))
  resmat = matrix(0, length(genes), length(genes))
  for(i in 1:length(genes)){
    for(j in 1:length(genes)){
      resmat[i,j] = length(intersect(genes[[i]], genes[[j]]))/length(genes[[i]])
    }
  }
  cl = hclust(dist(resmat), method = "ward.D2")
  cl = cutree(cl, ncl)
  res_df = data.frame("Description" = df$Description, "qvalue" = df$qvalue, "geneID" = df$geneID, 
                      cl, stringsAsFactors = F)
  res_df = res_df[order(res_df$qvalue, decreasing = F),]
  topterms = unlist(tapply(res_df$Description, res_df$cl, function(x) x[1:nt]))
  res_df = res_df[res_df$Description %in% topterms,]
  
  return(res_df)
}

Load data (from all cells)

getDPT = function(x){
  return(destiny:::dpt_for_branch(destiny::branch_divide(x, integer(0L)), 
                                  min(destiny::branch_divide(x, integer(0L))@branch[, 1], 
                                      na.rm = TRUE)))
}
CVgam = function (formula, data, nfold = 10, debug.level = 0, method = "glm.fit", 
    printit = TRUE, cvparts = NULL, seed = 29){
  # modified from the `gamclass` package to use gam::gam
    if (is.null(cvparts)) {
        set.seed(seed)
        cvparts <- sample(1:nfold, nrow(data), replace = TRUE)
    }
    folds <- unique(cvparts)
    khat <- hat <- numeric(nrow(data))
    scale.gam <- summary(gam::gam(formula, data = data, method = method,
                                  family = mgcv::betar(link = "logit", eps = 0.00001)))$scale
    for (i in folds) {
        trainrows <- cvparts != i
        testrows <- cvparts == i
        elev.gam <- gam::gam(formula, data = data[trainrows, ], 
                             method = method, 
                             family = mgcv::betar(link = "logit", eps = 0.00001))
        hat[testrows] <- predict(elev.gam, newdata = data[testrows,], select = T, type = "response")
        res <- residuals(elev.gam)
    }
    y <- eval(formula[[2]], envir = as.data.frame(data))
    res <- y - hat
    cvscale <- sum(res^2)/length(res)
    prntvec <- c(GAMscale = scale.gam, `CV-mse-GAM ` = cvscale)
    if (printit) 
        print(round(prntvec, 4))
    invisible(list(fitted = hat, resid = res, cvscale = cvscale, 
        scale.gam = scale.gam))
}
fittingFunc = function(g){
  t = sub_dat$zonation_pt
  gene_fit_p = c()
  gene_fit_vals = data.frame(row.names = 1:100)
  z = sub_dat@assays$SCT@data[g,]
    
  d = data.frame(z=z, t=t)
  tmp = suppressMessages(gam(z ~ ns(t, df = 3), data=d))
  
  # bins for model fitting
  bb = seq(min(t), max(t), length.out = 100)
  gene_fit_vals[,g] = suppressMessages(predict(tmp,
                                               newdata = data.frame(t = bb)))
  p = summary(tmp)$parametric.anova$`Pr(>F)`[1]
  gene_fit_p = c(gene_fit_p, p)
  names(gene_fit_p)[length(gene_fit_p)] = g
  
  return(list("fits" = gene_fit_vals, "pval" = gene_fit_p))
}
minmax_scale = function(x){
  return((x-min(x))/(max(x)-min(x)))
}
getTopTerms = function(godf, topt = 100, ncl = 5, nt = 2){
  topt = if(nrow(godf)<topt) nrow(godf) else topt
  if(nrow(godf)<ncl) return(godf)
  df = godf[1:topt,]
  genes = sapply(df$geneID, function(x) strsplit(x, "/"))
  resmat = matrix(0, length(genes), length(genes))
  for(i in 1:length(genes)){
    for(j in 1:length(genes)){
      resmat[i,j] = length(intersect(genes[[i]], genes[[j]]))/length(genes[[i]])
    }
  }
  cl = hclust(dist(resmat), method = "ward.D2")
  cl = cutree(cl, ncl)
  res_df = data.frame("Description" = df$Description, "qvalue" = df$qvalue, "geneID" = df$geneID, 
                      cl, stringsAsFactors = F)
  res_df = res_df[order(res_df$qvalue, decreasing = F),]
  topterms = unlist(tapply(res_df$Description, res_df$cl, function(x) x[1:nt]))
  res_df = res_df[res_df$Description %in% topterms,]
  
  return(res_df)
}

Endothelial cell clustering

Subset LSEC

allcells_css = readRDS(file = "data/processed/allcells_css.RDS")

Cluster and get markers

all_end_cells = allcells_css[,grepl("LSEC", allcells_css@meta.data$allcells_clusters)]
all_end_cells = suppressWarnings(SCTransform(all_end_cells, do.correct.umi = T, verbose = F, 
                                             vars.to.regress=c("unique_name","nCount_RNA"),
                                             variable.features.rv.th = 1, seed.use = 1,
                                             return.only.var.genes = F, 
                                             variable.features.n = NULL))
all_end_cells = RunPCA(all_end_cells, verbose = F)
all_end_cells = RunUMAP(all_end_cells, dims = 1:25, verbose = F)
DimPlot(all_end_cells, reduction = "umap", group.by = "Condition")

DimPlot(all_end_cells, reduction = "umap", group.by = "allcells_simp")

DimPlot(all_end_cells, reduction = "umap", group.by = "Donor")

DimPlot(all_end_cells, reduction = "umap", group.by = "Phase")

FeaturePlot(all_end_cells, reduction = "umap", features = c("MKI67", "ALB", "S100A8", "COLEC11",
                                                            "CLEC1B", "CLEC14A", "AQP1", "MGP", 
                                                            "LYVE1", "PROX1", "CD14", "PECAM1",
                                                            "VWF", "INMT", "PLVAP", "RBP7"))

Annotate the clusters

all_end_cells = FindNeighbors(all_end_cells, reduction = "pca", dims = 1:25,
                              prune.SNN = 1/5, force.recalc = T, graph.name = "pca25")
Computing nearest neighbor graph
Computing SNN
all_end_cells = FindClusters(all_end_cells, algorithm = 2, verbose = F, graph.name = "pca25",
                             resolution = seq(0.1, 2, 0.1))
DimPlot(all_end_cells, reduction = "umap", group.by = "pca25_res.1", label = T)
Using `as.character()` on a quosure is deprecated as of rlang 0.3.0.
Please use `as_label()` or `as_name()` instead.
This warning is displayed once per session.

DimPlot(all_end_cells, reduction = "umap", group.by = "Donor", label = F)

DimPlot(all_end_cells, reduction = "umap", group.by = "Condition", label = F)


all_end_cells = SetIdent(all_end_cells, value = "pca25_res.1")

Redo all w/o contaminating cells (only ECs of any type)

Markers for filtered clusters

DimPlot(only_end_cells, reduction = "umap", group.by = "pca25_res.0.9", label = T)
DimPlot(only_end_cells, reduction = "umap", group.by = "endo_simp", label = T)

DimPlot(only_end_cells, reduction = "umap", group.by = "Donor", label = F)

DimPlot(only_end_cells, reduction = "umap", group.by = "Condition", label = F)

Annotate the clusters for ECs only

annot = c("0" = "Midzonal LSEC",
"1" = "Pericentral LSEC",
"2" = "LSEC (stress)",
"3" = "Periportal LSEC",
"4" = "EC non-LSEC",
"5" = "LSEC (interferon)",
"6" = "LSEC (remodelling)",
"7" = "LSEC (high MT 1)",
"8" = "LSEC (high MT 2)",
"9" = "LSEC (fenestr.)",
"10" = "Lymphatic EC",
"11" = "Cycling cells",
"12" = "Periportal LSEC")

clnames = data.frame("endoOnly_annot" = only_end_cells@meta.data$pca25_res.0.9)
rownames(clnames) = colnames(only_end_cells)
clnames$endo_spec = plyr::revalue(clnames$endoOnly_annot, annot)
clnames$endo_simp = as.character(clnames$endo_spec)

only_end_cells = AddMetaData(only_end_cells, metadata = clnames)
DimPlot(only_end_cells, reduction = "umap", group.by = "endo_simp", label = F)

saveRDS(only_end_cells, file = "./results/endothelial/only_end_cells.RDS")

Get condition markers for each cluster

Plot abundance of clusters per condition

df_cnt = table(only_end_cells$endo_simp, only_end_cells$Condition)
df_cnt_perCond = reshape2::melt(apply(df_cnt, 2, function(x) round(x/sum(x)*100, 1)))
df_cnt_perCl = reshape2::melt(apply(df_cnt, 1, function(x) round(x/sum(x)*100, 1)))

mat_cnt_all = reshape2::dcast(data = df_cnt_perCond, formula = Var1 ~ Var2, value.var = "value")
rownames(mat_cnt_all) = mat_cnt_all$Var1
mat_cnt_all = mat_cnt_all[,-1]
ctord = hclust(dist(mat_cnt_all[,c(2,1,3)]))$order

heatp = pheatmap::pheatmap(mat_cnt_all[ctord,c(2,1,3)], cluster_cols = F, cluster_rows = F, 
                           treeheight_row = F, display_numbers = T, 
                           number_color = c("black","white")[as.integer(mat_cnt_all[ctord,c(2,1,3)]>25)+1],
                           color = colorRampPalette(brewer.pal(n = 9, name = "Blues"))(100),
                           fontsize_row = 8, fontsize_col = 8, angle_col = 0)

mat_cnt_all = reshape2::dcast(data = df_cnt_perCl, formula = Var1 ~ Var2, value.var = "value")
rownames(mat_cnt_all) = mat_cnt_all$Var1
mat_cnt_all = t(mat_cnt_all[,-1])
ctord = hclust(dist(mat_cnt_all[,c(2,1,3)]))$order

heatp = pheatmap::pheatmap(mat_cnt_all[ctord,c(2,1,3)], cluster_cols = F, cluster_rows = F, 
                           treeheight_row = F, display_numbers = T, 
                           number_color = c("black", "white")[as.integer(mat_cnt_all[ctord,c(2,1,3)]>50)+1],
                           color = colorRampPalette(brewer.pal(n = 9, name = "Blues"))(100),
                           fontsize_row = 8, fontsize_col = 8, angle_col = 0)

only_end_cells = SetIdent(only_end_cells, value = "endo_simp")
mkend_simp = FindAllMarkers(only_end_cells, logfc.threshold = 0.2, pseudocount.use = 0.1)
write.csv(mkend_simp[mkend_simp$p_val_adj<=0.05,], 
          file = "results/endothelial/markers_endo_subpop_simp.csv", row.names = T, quote = F)

Endothelial zonation

Make different subsets to test

df_cnt = table(only_end_cells$endo_simp, only_end_cells$Condition)
df_cnt_perCond = reshape2::melt(apply(df_cnt, 2, function(x) round(x/sum(x)*100, 1)))
df_cnt_perCl = reshape2::melt(apply(df_cnt, 1, function(x) round(x/sum(x)*100, 1)))

mat_cnt_all = reshape2::dcast(data = df_cnt_perCond, formula = Var1 ~ Var2, value.var = "value")
rownames(mat_cnt_all) = mat_cnt_all$Var1
mat_cnt_all = mat_cnt_all[,-1]
ctord = hclust(dist(mat_cnt_all[,c(2,1,3)]))$order

heatp = pheatmap::pheatmap(mat_cnt_all[ctord,c(2,1,3)], cluster_cols = F, cluster_rows = F, 
                           treeheight_row = F, display_numbers = T, 
                           number_color = c("black","white")[as.integer(mat_cnt_all[ctord,c(2,1,3)]>25)+1],
                           color = colorRampPalette(brewer.pal(n = 9, name = "Blues"))(100),
                           fontsize_row = 8, fontsize_col = 8, angle_col = 0)


mat_cnt_all = reshape2::dcast(data = df_cnt_perCl, formula = Var1 ~ Var2, value.var = "value")
rownames(mat_cnt_all) = mat_cnt_all$Var1
mat_cnt_all = t(mat_cnt_all[,-1])
ctord = hclust(dist(mat_cnt_all[,c(2,1,3)]))$order

heatp = pheatmap::pheatmap(mat_cnt_all[ctord,c(2,1,3)], cluster_cols = F, cluster_rows = F, 
                           treeheight_row = F, display_numbers = T, 
                           number_color = c("black", "white")[as.integer(mat_cnt_all[ctord,c(2,1,3)]>50)+1],
                           color = colorRampPalette(brewer.pal(n = 9, name = "Blues"))(100),
                           fontsize_row = 8, fontsize_col = 8, angle_col = 0)


only_end_cells = SetIdent(only_end_cells, value = "endo_simp")
mkend_simp = FindAllMarkers(only_end_cells, logfc.threshold = 0.2, pseudocount.use = 0.1)
Calculating cluster LSEC (stress)

  |                                                  | 0 % ~calculating  
  |+                                                 | 1 % ~27s          
  |++                                                | 2 % ~28s          
  |++                                                | 3 % ~28s          
  |+++                                               | 4 % ~27s          
  |+++                                               | 5 % ~27s          
  |++++                                              | 7 % ~27s          
  |++++                                              | 8 % ~27s          
  |+++++                                             | 9 % ~27s          
  |+++++                                             | 10% ~26s          
  |++++++                                            | 11% ~26s          
  |++++++                                            | 12% ~26s          
  |+++++++                                           | 13% ~25s          
  |++++++++                                          | 14% ~25s          
  |++++++++                                          | 15% ~25s          
  |+++++++++                                         | 16% ~24s          
  |+++++++++                                         | 17% ~24s          
  |++++++++++                                        | 18% ~24s          
  |++++++++++                                        | 20% ~23s          
  |+++++++++++                                       | 21% ~23s          
  |+++++++++++                                       | 22% ~23s          
  |++++++++++++                                      | 23% ~22s          
  |++++++++++++                                      | 24% ~22s          
  |+++++++++++++                                     | 25% ~22s          
  |++++++++++++++                                    | 26% ~21s          
  |++++++++++++++                                    | 27% ~21s          
  |+++++++++++++++                                   | 28% ~21s          
  |+++++++++++++++                                   | 29% ~21s          
  |++++++++++++++++                                  | 30% ~20s          
  |++++++++++++++++                                  | 32% ~20s          
  |+++++++++++++++++                                 | 33% ~20s          
  |+++++++++++++++++                                 | 34% ~19s          
  |++++++++++++++++++                                | 35% ~19s          
  |++++++++++++++++++                                | 36% ~19s          
  |+++++++++++++++++++                               | 37% ~18s          
  |++++++++++++++++++++                              | 38% ~18s          
  |++++++++++++++++++++                              | 39% ~18s          
  |+++++++++++++++++++++                             | 40% ~17s          
  |+++++++++++++++++++++                             | 41% ~17s          
  |++++++++++++++++++++++                            | 42% ~17s          
  |++++++++++++++++++++++                            | 43% ~16s          
  |+++++++++++++++++++++++                           | 45% ~16s          
  |+++++++++++++++++++++++                           | 46% ~16s          
  |++++++++++++++++++++++++                          | 47% ~15s          
  |++++++++++++++++++++++++                          | 48% ~15s          
  |+++++++++++++++++++++++++                         | 49% ~15s          
  |+++++++++++++++++++++++++                         | 50% ~15s          
  |++++++++++++++++++++++++++                        | 51% ~14s          
  |+++++++++++++++++++++++++++                       | 52% ~14s          
  |+++++++++++++++++++++++++++                       | 53% ~14s          
  |++++++++++++++++++++++++++++                      | 54% ~13s          
  |++++++++++++++++++++++++++++                      | 55% ~13s          
  |+++++++++++++++++++++++++++++                     | 57% ~13s          
  |+++++++++++++++++++++++++++++                     | 58% ~12s          
  |++++++++++++++++++++++++++++++                    | 59% ~12s          
  |++++++++++++++++++++++++++++++                    | 60% ~12s          
  |+++++++++++++++++++++++++++++++                   | 61% ~11s          
  |+++++++++++++++++++++++++++++++                   | 62% ~11s          
  |++++++++++++++++++++++++++++++++                  | 63% ~11s          
  |+++++++++++++++++++++++++++++++++                 | 64% ~10s          
  |+++++++++++++++++++++++++++++++++                 | 65% ~10s          
  |++++++++++++++++++++++++++++++++++                | 66% ~10s          
  |++++++++++++++++++++++++++++++++++                | 67% ~09s          
  |+++++++++++++++++++++++++++++++++++               | 68% ~09s          
  |+++++++++++++++++++++++++++++++++++               | 70% ~09s          
  |++++++++++++++++++++++++++++++++++++              | 71% ~09s          
  |++++++++++++++++++++++++++++++++++++              | 72% ~08s          
  |+++++++++++++++++++++++++++++++++++++             | 73% ~08s          
  |+++++++++++++++++++++++++++++++++++++             | 74% ~08s          
  |++++++++++++++++++++++++++++++++++++++            | 75% ~07s          
  |+++++++++++++++++++++++++++++++++++++++           | 76% ~07s          
  |+++++++++++++++++++++++++++++++++++++++           | 77% ~07s          
  |++++++++++++++++++++++++++++++++++++++++          | 78% ~06s          
  |++++++++++++++++++++++++++++++++++++++++          | 79% ~06s          
  |+++++++++++++++++++++++++++++++++++++++++         | 80% ~06s          
  |+++++++++++++++++++++++++++++++++++++++++         | 82% ~05s          
  |++++++++++++++++++++++++++++++++++++++++++        | 83% ~05s          
  |++++++++++++++++++++++++++++++++++++++++++        | 84% ~05s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~04s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 86% ~04s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~04s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 88% ~03s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~03s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 90% ~03s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~03s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 92% ~02s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~02s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~02s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=29s  
Calculating cluster EC non-LSEC

  |                                                  | 0 % ~calculating  
  |+                                                 | 1 % ~01m 14s      
  |++                                                | 2 % ~01m 15s      
  |++                                                | 3 % ~01m 14s      
  |+++                                               | 4 % ~01m 13s      
  |+++                                               | 5 % ~01m 13s      
  |++++                                              | 6 % ~01m 12s      
  |++++                                              | 7 % ~01m 11s      
  |+++++                                             | 8 % ~01m 10s      
  |+++++                                             | 9 % ~01m 09s      
  |++++++                                            | 10% ~01m 08s      
  |++++++                                            | 11% ~01m 07s      
  |+++++++                                           | 12% ~01m 07s      
  |+++++++                                           | 13% ~01m 06s      
  |++++++++                                          | 14% ~01m 05s      
  |++++++++                                          | 15% ~01m 04s      
  |+++++++++                                         | 16% ~01m 04s      
  |+++++++++                                         | 17% ~01m 03s      
  |++++++++++                                        | 18% ~01m 02s      
  |++++++++++                                        | 19% ~01m 01s      
  |+++++++++++                                       | 20% ~01m 01s      
  |+++++++++++                                       | 21% ~60s          
  |++++++++++++                                      | 22% ~59s          
  |++++++++++++                                      | 23% ~58s          
  |+++++++++++++                                     | 24% ~58s          
  |+++++++++++++                                     | 26% ~57s          
  |++++++++++++++                                    | 27% ~56s          
  |++++++++++++++                                    | 28% ~55s          
  |+++++++++++++++                                   | 29% ~54s          
  |+++++++++++++++                                   | 30% ~54s          
  |++++++++++++++++                                  | 31% ~53s          
  |++++++++++++++++                                  | 32% ~52s          
  |+++++++++++++++++                                 | 33% ~51s          
  |+++++++++++++++++                                 | 34% ~51s          
  |++++++++++++++++++                                | 35% ~50s          
  |++++++++++++++++++                                | 36% ~49s          
  |+++++++++++++++++++                               | 37% ~48s          
  |+++++++++++++++++++                               | 38% ~47s          
  |++++++++++++++++++++                              | 39% ~47s          
  |++++++++++++++++++++                              | 40% ~46s          
  |+++++++++++++++++++++                             | 41% ~45s          
  |+++++++++++++++++++++                             | 42% ~44s          
  |++++++++++++++++++++++                            | 43% ~44s          
  |++++++++++++++++++++++                            | 44% ~43s          
  |+++++++++++++++++++++++                           | 45% ~42s          
  |+++++++++++++++++++++++                           | 46% ~41s          
  |++++++++++++++++++++++++                          | 47% ~41s          
  |++++++++++++++++++++++++                          | 48% ~40s          
  |+++++++++++++++++++++++++                         | 49% ~39s          
  |+++++++++++++++++++++++++                         | 50% ~38s          
  |++++++++++++++++++++++++++                        | 51% ~37s          
  |+++++++++++++++++++++++++++                       | 52% ~37s          
  |+++++++++++++++++++++++++++                       | 53% ~36s          
  |++++++++++++++++++++++++++++                      | 54% ~35s          
  |++++++++++++++++++++++++++++                      | 55% ~34s          
  |+++++++++++++++++++++++++++++                     | 56% ~34s          
  |+++++++++++++++++++++++++++++                     | 57% ~33s          
  |++++++++++++++++++++++++++++++                    | 58% ~32s          
  |++++++++++++++++++++++++++++++                    | 59% ~31s          
  |+++++++++++++++++++++++++++++++                   | 60% ~30s          
  |+++++++++++++++++++++++++++++++                   | 61% ~30s          
  |++++++++++++++++++++++++++++++++                  | 62% ~29s          
  |++++++++++++++++++++++++++++++++                  | 63% ~28s          
  |+++++++++++++++++++++++++++++++++                 | 64% ~27s          
  |+++++++++++++++++++++++++++++++++                 | 65% ~27s          
  |++++++++++++++++++++++++++++++++++                | 66% ~26s          
  |++++++++++++++++++++++++++++++++++                | 67% ~25s          
  |+++++++++++++++++++++++++++++++++++               | 68% ~24s          
  |+++++++++++++++++++++++++++++++++++               | 69% ~23s          
  |++++++++++++++++++++++++++++++++++++              | 70% ~23s          
  |++++++++++++++++++++++++++++++++++++              | 71% ~22s          
  |+++++++++++++++++++++++++++++++++++++             | 72% ~21s          
  |+++++++++++++++++++++++++++++++++++++             | 73% ~20s          
  |++++++++++++++++++++++++++++++++++++++            | 74% ~20s          
  |++++++++++++++++++++++++++++++++++++++            | 76% ~19s          
  |+++++++++++++++++++++++++++++++++++++++           | 77% ~18s          
  |+++++++++++++++++++++++++++++++++++++++           | 78% ~17s          
  |++++++++++++++++++++++++++++++++++++++++          | 79% ~16s          
  |++++++++++++++++++++++++++++++++++++++++          | 80% ~16s          
  |+++++++++++++++++++++++++++++++++++++++++         | 81% ~15s          
  |+++++++++++++++++++++++++++++++++++++++++         | 82% ~14s          
  |++++++++++++++++++++++++++++++++++++++++++        | 83% ~13s          
  |++++++++++++++++++++++++++++++++++++++++++        | 84% ~13s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~12s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 86% ~11s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~10s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 88% ~09s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~09s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 90% ~08s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~07s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 92% ~06s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~05s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 94% ~05s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~04s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~03s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~02s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~02s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=01m 16s
Calculating cluster LSEC (remodelling)

  |                                                  | 0 % ~calculating  
  |+                                                 | 1 % ~33s          
  |++                                                | 2 % ~32s          
  |++                                                | 3 % ~33s          
  |+++                                               | 4 % ~33s          
  |+++                                               | 5 % ~33s          
  |++++                                              | 6 % ~33s          
  |++++                                              | 7 % ~32s          
  |+++++                                             | 8 % ~32s          
  |+++++                                             | 9 % ~32s          
  |++++++                                            | 10% ~31s          
  |++++++                                            | 11% ~31s          
  |+++++++                                           | 12% ~31s          
  |+++++++                                           | 13% ~31s          
  |++++++++                                          | 14% ~30s          
  |++++++++                                          | 15% ~30s          
  |+++++++++                                         | 16% ~30s          
  |+++++++++                                         | 17% ~31s          
  |++++++++++                                        | 18% ~30s          
  |++++++++++                                        | 19% ~30s          
  |+++++++++++                                       | 20% ~29s          
  |+++++++++++                                       | 21% ~29s          
  |++++++++++++                                      | 22% ~28s          
  |++++++++++++                                      | 23% ~28s          
  |+++++++++++++                                     | 24% ~28s          
  |+++++++++++++                                     | 25% ~27s          
  |++++++++++++++                                    | 26% ~27s          
  |++++++++++++++                                    | 27% ~26s          
  |+++++++++++++++                                   | 28% ~26s          
  |+++++++++++++++                                   | 29% ~25s          
  |++++++++++++++++                                  | 30% ~25s          
  |++++++++++++++++                                  | 31% ~25s          
  |+++++++++++++++++                                 | 32% ~24s          
  |+++++++++++++++++                                 | 33% ~24s          
  |++++++++++++++++++                                | 34% ~24s          
  |++++++++++++++++++                                | 35% ~23s          
  |+++++++++++++++++++                               | 36% ~23s          
  |+++++++++++++++++++                               | 37% ~22s          
  |++++++++++++++++++++                              | 38% ~22s          
  |++++++++++++++++++++                              | 39% ~22s          
  |+++++++++++++++++++++                             | 40% ~21s          
  |+++++++++++++++++++++                             | 41% ~21s          
  |++++++++++++++++++++++                            | 42% ~21s          
  |++++++++++++++++++++++                            | 43% ~20s          
  |+++++++++++++++++++++++                           | 44% ~20s          
  |+++++++++++++++++++++++                           | 45% ~20s          
  |++++++++++++++++++++++++                          | 46% ~19s          
  |++++++++++++++++++++++++                          | 47% ~19s          
  |+++++++++++++++++++++++++                         | 48% ~19s          
  |+++++++++++++++++++++++++                         | 49% ~18s          
  |++++++++++++++++++++++++++                        | 51% ~18s          
  |++++++++++++++++++++++++++                        | 52% ~18s          
  |+++++++++++++++++++++++++++                       | 53% ~17s          
  |+++++++++++++++++++++++++++                       | 54% ~17s          
  |++++++++++++++++++++++++++++                      | 55% ~17s          
  |++++++++++++++++++++++++++++                      | 56% ~16s          
  |+++++++++++++++++++++++++++++                     | 57% ~16s          
  |+++++++++++++++++++++++++++++                     | 58% ~15s          
  |++++++++++++++++++++++++++++++                    | 59% ~15s          
  |++++++++++++++++++++++++++++++                    | 60% ~15s          
  |+++++++++++++++++++++++++++++++                   | 61% ~14s          
  |+++++++++++++++++++++++++++++++                   | 62% ~14s          
  |++++++++++++++++++++++++++++++++                  | 63% ~14s          
  |++++++++++++++++++++++++++++++++                  | 64% ~13s          
  |+++++++++++++++++++++++++++++++++                 | 65% ~13s          
  |+++++++++++++++++++++++++++++++++                 | 66% ~12s          
  |++++++++++++++++++++++++++++++++++                | 67% ~12s          
  |++++++++++++++++++++++++++++++++++                | 68% ~12s          
  |+++++++++++++++++++++++++++++++++++               | 69% ~11s          
  |+++++++++++++++++++++++++++++++++++               | 70% ~11s          
  |++++++++++++++++++++++++++++++++++++              | 71% ~11s          
  |++++++++++++++++++++++++++++++++++++              | 72% ~10s          
  |+++++++++++++++++++++++++++++++++++++             | 73% ~10s          
  |+++++++++++++++++++++++++++++++++++++             | 74% ~09s          
  |++++++++++++++++++++++++++++++++++++++            | 75% ~09s          
  |++++++++++++++++++++++++++++++++++++++            | 76% ~09s          
  |+++++++++++++++++++++++++++++++++++++++           | 77% ~08s          
  |+++++++++++++++++++++++++++++++++++++++           | 78% ~08s          
  |++++++++++++++++++++++++++++++++++++++++          | 79% ~08s          
  |++++++++++++++++++++++++++++++++++++++++          | 80% ~07s          
  |+++++++++++++++++++++++++++++++++++++++++         | 81% ~07s          
  |+++++++++++++++++++++++++++++++++++++++++         | 82% ~07s          
  |++++++++++++++++++++++++++++++++++++++++++        | 83% ~06s          
  |++++++++++++++++++++++++++++++++++++++++++        | 84% ~06s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~05s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 86% ~05s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~05s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 88% ~04s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~04s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 90% ~04s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~03s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 92% ~03s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~03s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 94% ~02s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~02s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=36s  
Calculating cluster Pericentral LSEC

  |                                                  | 0 % ~calculating  
  |+                                                 | 1 % ~49s          
  |++                                                | 2 % ~50s          
  |++                                                | 3 % ~48s          
  |+++                                               | 4 % ~49s          
  |+++                                               | 5 % ~51s          
  |++++                                              | 6 % ~52s          
  |++++                                              | 7 % ~53s          
  |+++++                                             | 8 % ~53s          
  |+++++                                             | 9 % ~53s          
  |++++++                                            | 10% ~53s          
  |++++++                                            | 11% ~51s          
  |+++++++                                           | 12% ~50s          
  |+++++++                                           | 13% ~49s          
  |++++++++                                          | 14% ~48s          
  |++++++++                                          | 15% ~47s          
  |+++++++++                                         | 16% ~46s          
  |+++++++++                                         | 17% ~46s          
  |++++++++++                                        | 18% ~45s          
  |++++++++++                                        | 19% ~44s          
  |+++++++++++                                       | 20% ~43s          
  |+++++++++++                                       | 21% ~43s          
  |++++++++++++                                      | 22% ~42s          
  |++++++++++++                                      | 23% ~41s          
  |+++++++++++++                                     | 24% ~41s          
  |+++++++++++++                                     | 26% ~40s          
  |++++++++++++++                                    | 27% ~39s          
  |++++++++++++++                                    | 28% ~38s          
  |+++++++++++++++                                   | 29% ~38s          
  |+++++++++++++++                                   | 30% ~37s          
  |++++++++++++++++                                  | 31% ~37s          
  |++++++++++++++++                                  | 32% ~36s          
  |+++++++++++++++++                                 | 33% ~35s          
  |+++++++++++++++++                                 | 34% ~35s          
  |++++++++++++++++++                                | 35% ~34s          
  |++++++++++++++++++                                | 36% ~34s          
  |+++++++++++++++++++                               | 37% ~33s          
  |+++++++++++++++++++                               | 38% ~33s          
  |++++++++++++++++++++                              | 39% ~32s          
  |++++++++++++++++++++                              | 40% ~31s          
  |+++++++++++++++++++++                             | 41% ~31s          
  |+++++++++++++++++++++                             | 42% ~30s          
  |++++++++++++++++++++++                            | 43% ~30s          
  |++++++++++++++++++++++                            | 44% ~29s          
  |+++++++++++++++++++++++                           | 45% ~29s          
  |+++++++++++++++++++++++                           | 46% ~28s          
  |++++++++++++++++++++++++                          | 47% ~27s          
  |++++++++++++++++++++++++                          | 48% ~27s          
  |+++++++++++++++++++++++++                         | 49% ~26s          
  |+++++++++++++++++++++++++                         | 50% ~26s          
  |++++++++++++++++++++++++++                        | 51% ~25s          
  |+++++++++++++++++++++++++++                       | 52% ~25s          
  |+++++++++++++++++++++++++++                       | 53% ~24s          
  |++++++++++++++++++++++++++++                      | 54% ~24s          
  |++++++++++++++++++++++++++++                      | 55% ~23s          
  |+++++++++++++++++++++++++++++                     | 56% ~23s          
  |+++++++++++++++++++++++++++++                     | 57% ~22s          
  |++++++++++++++++++++++++++++++                    | 58% ~22s          
  |++++++++++++++++++++++++++++++                    | 59% ~21s          
  |+++++++++++++++++++++++++++++++                   | 60% ~20s          
  |+++++++++++++++++++++++++++++++                   | 61% ~20s          
  |++++++++++++++++++++++++++++++++                  | 62% ~19s          
  |++++++++++++++++++++++++++++++++                  | 63% ~19s          
  |+++++++++++++++++++++++++++++++++                 | 64% ~18s          
  |+++++++++++++++++++++++++++++++++                 | 65% ~18s          
  |++++++++++++++++++++++++++++++++++                | 66% ~17s          
  |++++++++++++++++++++++++++++++++++                | 67% ~17s          
  |+++++++++++++++++++++++++++++++++++               | 68% ~16s          
  |+++++++++++++++++++++++++++++++++++               | 69% ~16s          
  |++++++++++++++++++++++++++++++++++++              | 70% ~15s          
  |++++++++++++++++++++++++++++++++++++              | 71% ~15s          
  |+++++++++++++++++++++++++++++++++++++             | 72% ~14s          
  |+++++++++++++++++++++++++++++++++++++             | 73% ~14s          
  |++++++++++++++++++++++++++++++++++++++            | 74% ~13s          
  |++++++++++++++++++++++++++++++++++++++            | 76% ~13s          
  |+++++++++++++++++++++++++++++++++++++++           | 77% ~12s          
  |+++++++++++++++++++++++++++++++++++++++           | 78% ~11s          
  |++++++++++++++++++++++++++++++++++++++++          | 79% ~11s          
  |++++++++++++++++++++++++++++++++++++++++          | 80% ~10s          
  |+++++++++++++++++++++++++++++++++++++++++         | 81% ~10s          
  |+++++++++++++++++++++++++++++++++++++++++         | 82% ~09s          
  |++++++++++++++++++++++++++++++++++++++++++        | 83% ~09s          
  |++++++++++++++++++++++++++++++++++++++++++        | 84% ~08s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~08s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 86% ~07s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~07s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 88% ~06s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~06s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 90% ~05s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~05s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 92% ~04s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~04s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 94% ~03s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~03s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~02s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~02s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=51s  
Calculating cluster LSEC (high MT 2)

  |                                                  | 0 % ~calculating  
  |+                                                 | 1 % ~02m 22s      
  |++                                                | 2 % ~02m 24s      
  |++                                                | 3 % ~02m 22s      
  |+++                                               | 4 % ~02m 21s      
  |+++                                               | 5 % ~02m 19s      
  |++++                                              | 6 % ~02m 18s      
  |++++                                              | 7 % ~02m 16s      
  |+++++                                             | 8 % ~02m 15s      
  |+++++                                             | 9 % ~02m 13s      
  |++++++                                            | 10% ~02m 12s      
  |++++++                                            | 11% ~02m 10s      
  |+++++++                                           | 12% ~02m 08s      
  |+++++++                                           | 13% ~02m 07s      
  |++++++++                                          | 14% ~02m 06s      
  |++++++++                                          | 15% ~02m 04s      
  |+++++++++                                         | 16% ~02m 03s      
  |+++++++++                                         | 17% ~02m 01s      
  |++++++++++                                        | 18% ~01m 60s      
  |++++++++++                                        | 19% ~01m 58s      
  |+++++++++++                                       | 20% ~01m 57s      
  |+++++++++++                                       | 21% ~01m 55s      
  |++++++++++++                                      | 22% ~01m 54s      
  |++++++++++++                                      | 23% ~01m 52s      
  |+++++++++++++                                     | 24% ~01m 51s      
  |+++++++++++++                                     | 25% ~01m 51s      
  |++++++++++++++                                    | 26% ~01m 50s      
  |++++++++++++++                                    | 27% ~01m 48s      
  |+++++++++++++++                                   | 28% ~01m 47s      
  |+++++++++++++++                                   | 29% ~01m 45s      
  |++++++++++++++++                                  | 30% ~01m 44s      
  |++++++++++++++++                                  | 31% ~01m 42s      
  |+++++++++++++++++                                 | 32% ~01m 41s      
  |+++++++++++++++++                                 | 33% ~01m 39s      
  |++++++++++++++++++                                | 34% ~01m 38s      
  |++++++++++++++++++                                | 35% ~01m 36s      
  |+++++++++++++++++++                               | 36% ~01m 34s      
  |+++++++++++++++++++                               | 37% ~01m 33s      
  |++++++++++++++++++++                              | 38% ~01m 31s      
  |++++++++++++++++++++                              | 39% ~01m 30s      
  |+++++++++++++++++++++                             | 40% ~01m 28s      
  |+++++++++++++++++++++                             | 41% ~01m 27s      
  |++++++++++++++++++++++                            | 42% ~01m 25s      
  |++++++++++++++++++++++                            | 43% ~01m 24s      
  |+++++++++++++++++++++++                           | 44% ~01m 22s      
  |+++++++++++++++++++++++                           | 45% ~01m 21s      
  |++++++++++++++++++++++++                          | 46% ~01m 19s      
  |++++++++++++++++++++++++                          | 47% ~01m 18s      
  |+++++++++++++++++++++++++                         | 48% ~01m 16s      
  |+++++++++++++++++++++++++                         | 49% ~01m 15s      
  |++++++++++++++++++++++++++                        | 51% ~01m 13s      
  |++++++++++++++++++++++++++                        | 52% ~01m 12s      
  |+++++++++++++++++++++++++++                       | 53% ~01m 10s      
  |+++++++++++++++++++++++++++                       | 54% ~01m 09s      
  |++++++++++++++++++++++++++++                      | 55% ~01m 07s      
  |++++++++++++++++++++++++++++                      | 56% ~01m 06s      
  |+++++++++++++++++++++++++++++                     | 57% ~01m 04s      
  |+++++++++++++++++++++++++++++                     | 58% ~01m 03s      
  |++++++++++++++++++++++++++++++                    | 59% ~01m 01s      
  |++++++++++++++++++++++++++++++                    | 60% ~60s          
  |+++++++++++++++++++++++++++++++                   | 61% ~58s          
  |+++++++++++++++++++++++++++++++                   | 62% ~57s          
  |++++++++++++++++++++++++++++++++                  | 63% ~55s          
  |++++++++++++++++++++++++++++++++                  | 64% ~54s          
  |+++++++++++++++++++++++++++++++++                 | 65% ~52s          
  |+++++++++++++++++++++++++++++++++                 | 66% ~51s          
  |++++++++++++++++++++++++++++++++++                | 67% ~49s          
  |++++++++++++++++++++++++++++++++++                | 68% ~48s          
  |+++++++++++++++++++++++++++++++++++               | 69% ~46s          
  |+++++++++++++++++++++++++++++++++++               | 70% ~45s          
  |++++++++++++++++++++++++++++++++++++              | 71% ~43s          
  |++++++++++++++++++++++++++++++++++++              | 72% ~42s          
  |+++++++++++++++++++++++++++++++++++++             | 73% ~40s          
  |+++++++++++++++++++++++++++++++++++++             | 74% ~39s          
  |++++++++++++++++++++++++++++++++++++++            | 75% ~37s          
  |++++++++++++++++++++++++++++++++++++++            | 76% ~36s          
  |+++++++++++++++++++++++++++++++++++++++           | 77% ~34s          
  |+++++++++++++++++++++++++++++++++++++++           | 78% ~33s          
  |++++++++++++++++++++++++++++++++++++++++          | 79% ~31s          
  |++++++++++++++++++++++++++++++++++++++++          | 80% ~30s          
  |+++++++++++++++++++++++++++++++++++++++++         | 81% ~28s          
  |+++++++++++++++++++++++++++++++++++++++++         | 82% ~27s          
  |++++++++++++++++++++++++++++++++++++++++++        | 83% ~25s          
  |++++++++++++++++++++++++++++++++++++++++++        | 84% ~24s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~22s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 86% ~21s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~19s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 88% ~18s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~16s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 90% ~15s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~13s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 92% ~12s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~10s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 94% ~09s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~07s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~06s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~04s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~03s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=02m 27s
Calculating cluster Midzonal LSEC

  |                                                  | 0 % ~calculating  
  |+                                                 | 1 % ~24s          
  |++                                                | 2 % ~24s          
  |++                                                | 3 % ~24s          
  |+++                                               | 4 % ~24s          
  |+++                                               | 5 % ~24s          
  |++++                                              | 6 % ~24s          
  |++++                                              | 7 % ~24s          
  |+++++                                             | 8 % ~23s          
  |+++++                                             | 9 % ~23s          
  |++++++                                            | 10% ~23s          
  |++++++                                            | 11% ~23s          
  |+++++++                                           | 12% ~22s          
  |+++++++                                           | 13% ~22s          
  |++++++++                                          | 14% ~22s          
  |++++++++                                          | 15% ~22s          
  |+++++++++                                         | 16% ~21s          
  |+++++++++                                         | 17% ~21s          
  |++++++++++                                        | 18% ~21s          
  |++++++++++                                        | 19% ~20s          
  |+++++++++++                                       | 20% ~20s          
  |+++++++++++                                       | 21% ~20s          
  |++++++++++++                                      | 22% ~20s          
  |++++++++++++                                      | 23% ~19s          
  |+++++++++++++                                     | 24% ~19s          
  |+++++++++++++                                     | 25% ~19s          
  |++++++++++++++                                    | 26% ~19s          
  |++++++++++++++                                    | 27% ~18s          
  |+++++++++++++++                                   | 28% ~18s          
  |+++++++++++++++                                   | 29% ~18s          
  |++++++++++++++++                                  | 30% ~18s          
  |++++++++++++++++                                  | 31% ~17s          
  |+++++++++++++++++                                 | 32% ~17s          
  |+++++++++++++++++                                 | 33% ~17s          
  |++++++++++++++++++                                | 34% ~17s          
  |++++++++++++++++++                                | 35% ~16s          
  |+++++++++++++++++++                               | 36% ~16s          
  |+++++++++++++++++++                               | 37% ~16s          
  |++++++++++++++++++++                              | 38% ~16s          
  |++++++++++++++++++++                              | 39% ~15s          
  |+++++++++++++++++++++                             | 40% ~15s          
  |+++++++++++++++++++++                             | 41% ~15s          
  |++++++++++++++++++++++                            | 42% ~15s          
  |++++++++++++++++++++++                            | 43% ~14s          
  |+++++++++++++++++++++++                           | 44% ~14s          
  |+++++++++++++++++++++++                           | 45% ~14s          
  |++++++++++++++++++++++++                          | 46% ~14s          
  |++++++++++++++++++++++++                          | 47% ~13s          
  |+++++++++++++++++++++++++                         | 48% ~13s          
  |+++++++++++++++++++++++++                         | 49% ~13s          
  |++++++++++++++++++++++++++                        | 51% ~13s          
  |++++++++++++++++++++++++++                        | 52% ~12s          
  |+++++++++++++++++++++++++++                       | 53% ~12s          
  |+++++++++++++++++++++++++++                       | 54% ~12s          
  |++++++++++++++++++++++++++++                      | 55% ~12s          
  |++++++++++++++++++++++++++++                      | 56% ~11s          
  |+++++++++++++++++++++++++++++                     | 57% ~11s          
  |+++++++++++++++++++++++++++++                     | 58% ~11s          
  |++++++++++++++++++++++++++++++                    | 59% ~11s          
  |++++++++++++++++++++++++++++++                    | 60% ~10s          
  |+++++++++++++++++++++++++++++++                   | 61% ~10s          
  |+++++++++++++++++++++++++++++++                   | 62% ~10s          
  |++++++++++++++++++++++++++++++++                  | 63% ~10s          
  |++++++++++++++++++++++++++++++++                  | 64% ~09s          
  |+++++++++++++++++++++++++++++++++                 | 65% ~09s          
  |+++++++++++++++++++++++++++++++++                 | 66% ~09s          
  |++++++++++++++++++++++++++++++++++                | 67% ~09s          
  |++++++++++++++++++++++++++++++++++                | 68% ~08s          
  |+++++++++++++++++++++++++++++++++++               | 69% ~08s          
  |+++++++++++++++++++++++++++++++++++               | 70% ~08s          
  |++++++++++++++++++++++++++++++++++++              | 71% ~07s          
  |++++++++++++++++++++++++++++++++++++              | 72% ~07s          
  |+++++++++++++++++++++++++++++++++++++             | 73% ~07s          
  |+++++++++++++++++++++++++++++++++++++             | 74% ~07s          
  |++++++++++++++++++++++++++++++++++++++            | 75% ~06s          
  |++++++++++++++++++++++++++++++++++++++            | 76% ~06s          
  |+++++++++++++++++++++++++++++++++++++++           | 77% ~06s          
  |+++++++++++++++++++++++++++++++++++++++           | 78% ~06s          
  |++++++++++++++++++++++++++++++++++++++++          | 79% ~05s          
  |++++++++++++++++++++++++++++++++++++++++          | 80% ~05s          
  |+++++++++++++++++++++++++++++++++++++++++         | 81% ~05s          
  |+++++++++++++++++++++++++++++++++++++++++         | 82% ~05s          
  |++++++++++++++++++++++++++++++++++++++++++        | 83% ~04s          
  |++++++++++++++++++++++++++++++++++++++++++        | 84% ~04s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~04s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 86% ~04s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~03s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 88% ~03s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~03s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 90% ~03s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~02s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 92% ~02s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~02s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 94% ~02s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=26s  
Calculating cluster LSEC (high MT 1)

  |                                                  | 0 % ~calculating  
  |+                                                 | 1 % ~02m 19s      
  |++                                                | 2 % ~02m 18s      
  |++                                                | 3 % ~02m 16s      
  |+++                                               | 4 % ~02m 16s      
  |+++                                               | 5 % ~02m 14s      
  |++++                                              | 6 % ~02m 13s      
  |++++                                              | 7 % ~02m 11s      
  |+++++                                             | 8 % ~02m 09s      
  |+++++                                             | 9 % ~02m 08s      
  |++++++                                            | 10% ~02m 06s      
  |++++++                                            | 11% ~02m 05s      
  |+++++++                                           | 12% ~02m 03s      
  |+++++++                                           | 13% ~02m 02s      
  |++++++++                                          | 14% ~02m 00s      
  |++++++++                                          | 15% ~01m 59s      
  |+++++++++                                         | 16% ~01m 57s      
  |+++++++++                                         | 18% ~01m 56s      
  |++++++++++                                        | 19% ~01m 54s      
  |++++++++++                                        | 20% ~01m 53s      
  |+++++++++++                                       | 21% ~01m 51s      
  |+++++++++++                                       | 22% ~01m 50s      
  |++++++++++++                                      | 23% ~01m 49s      
  |++++++++++++                                      | 24% ~01m 47s      
  |+++++++++++++                                     | 25% ~01m 46s      
  |+++++++++++++                                     | 26% ~01m 44s      
  |++++++++++++++                                    | 27% ~01m 43s      
  |++++++++++++++                                    | 28% ~01m 41s      
  |+++++++++++++++                                   | 29% ~01m 40s      
  |+++++++++++++++                                   | 30% ~01m 38s      
  |++++++++++++++++                                  | 31% ~01m 37s      
  |++++++++++++++++                                  | 32% ~01m 35s      
  |+++++++++++++++++                                 | 33% ~01m 34s      
  |++++++++++++++++++                                | 34% ~01m 33s      
  |++++++++++++++++++                                | 35% ~01m 31s      
  |+++++++++++++++++++                               | 36% ~01m 30s      
  |+++++++++++++++++++                               | 37% ~01m 28s      
  |++++++++++++++++++++                              | 38% ~01m 27s      
  |++++++++++++++++++++                              | 39% ~01m 25s      
  |+++++++++++++++++++++                             | 40% ~01m 24s      
  |+++++++++++++++++++++                             | 41% ~01m 23s      
  |++++++++++++++++++++++                            | 42% ~01m 21s      
  |++++++++++++++++++++++                            | 43% ~01m 20s      
  |+++++++++++++++++++++++                           | 44% ~01m 18s      
  |+++++++++++++++++++++++                           | 45% ~01m 17s      
  |++++++++++++++++++++++++                          | 46% ~01m 16s      
  |++++++++++++++++++++++++                          | 47% ~01m 14s      
  |+++++++++++++++++++++++++                         | 48% ~01m 13s      
  |+++++++++++++++++++++++++                         | 49% ~01m 11s      
  |++++++++++++++++++++++++++                        | 51% ~01m 10s      
  |++++++++++++++++++++++++++                        | 52% ~01m 08s      
  |+++++++++++++++++++++++++++                       | 53% ~01m 07s      
  |+++++++++++++++++++++++++++                       | 54% ~01m 05s      
  |++++++++++++++++++++++++++++                      | 55% ~01m 04s      
  |++++++++++++++++++++++++++++                      | 56% ~01m 02s      
  |+++++++++++++++++++++++++++++                     | 57% ~01m 01s      
  |+++++++++++++++++++++++++++++                     | 58% ~60s          
  |++++++++++++++++++++++++++++++                    | 59% ~58s          
  |++++++++++++++++++++++++++++++                    | 60% ~57s          
  |+++++++++++++++++++++++++++++++                   | 61% ~55s          
  |+++++++++++++++++++++++++++++++                   | 62% ~54s          
  |++++++++++++++++++++++++++++++++                  | 63% ~52s          
  |++++++++++++++++++++++++++++++++                  | 64% ~51s          
  |+++++++++++++++++++++++++++++++++                 | 65% ~49s          
  |+++++++++++++++++++++++++++++++++                 | 66% ~48s          
  |++++++++++++++++++++++++++++++++++                | 67% ~47s          
  |+++++++++++++++++++++++++++++++++++               | 68% ~45s          
  |+++++++++++++++++++++++++++++++++++               | 69% ~44s          
  |++++++++++++++++++++++++++++++++++++              | 70% ~42s          
  |++++++++++++++++++++++++++++++++++++              | 71% ~41s          
  |+++++++++++++++++++++++++++++++++++++             | 72% ~40s          
  |+++++++++++++++++++++++++++++++++++++             | 73% ~38s          
  |++++++++++++++++++++++++++++++++++++++            | 74% ~37s          
  |++++++++++++++++++++++++++++++++++++++            | 75% ~36s          
  |+++++++++++++++++++++++++++++++++++++++           | 76% ~34s          
  |+++++++++++++++++++++++++++++++++++++++           | 77% ~33s          
  |++++++++++++++++++++++++++++++++++++++++          | 78% ~31s          
  |++++++++++++++++++++++++++++++++++++++++          | 79% ~30s          
  |+++++++++++++++++++++++++++++++++++++++++         | 80% ~28s          
  |+++++++++++++++++++++++++++++++++++++++++         | 81% ~27s          
  |++++++++++++++++++++++++++++++++++++++++++        | 82% ~25s          
  |++++++++++++++++++++++++++++++++++++++++++        | 84% ~24s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~22s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 86% ~21s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~19s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 88% ~18s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~16s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 90% ~15s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~13s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 92% ~12s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~10s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 94% ~09s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~07s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~06s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~04s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~03s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=02m 23s
Calculating cluster LSEC (interferon)

  |                                                  | 0 % ~calculating  
  |+                                                 | 1 % ~03m 12s      
  |++                                                | 2 % ~03m 12s      
  |++                                                | 3 % ~03m 11s      
  |+++                                               | 4 % ~03m 09s      
  |+++                                               | 5 % ~03m 06s      
  |++++                                              | 6 % ~03m 04s      
  |++++                                              | 7 % ~03m 02s      
  |+++++                                             | 8 % ~03m 00s      
  |+++++                                             | 9 % ~02m 58s      
  |++++++                                            | 10% ~02m 56s      
  |++++++                                            | 11% ~02m 54s      
  |+++++++                                           | 12% ~02m 52s      
  |+++++++                                           | 13% ~02m 50s      
  |++++++++                                          | 14% ~02m 48s      
  |++++++++                                          | 15% ~02m 46s      
  |+++++++++                                         | 16% ~02m 44s      
  |+++++++++                                         | 17% ~02m 42s      
  |++++++++++                                        | 18% ~02m 40s      
  |++++++++++                                        | 19% ~02m 39s      
  |+++++++++++                                       | 20% ~02m 36s      
  |+++++++++++                                       | 21% ~02m 35s      
  |++++++++++++                                      | 22% ~02m 33s      
  |++++++++++++                                      | 23% ~02m 31s      
  |+++++++++++++                                     | 24% ~02m 29s      
  |+++++++++++++                                     | 26% ~02m 27s      
  |++++++++++++++                                    | 27% ~02m 25s      
  |++++++++++++++                                    | 28% ~02m 23s      
  |+++++++++++++++                                   | 29% ~02m 21s      
  |+++++++++++++++                                   | 30% ~02m 19s      
  |++++++++++++++++                                  | 31% ~02m 17s      
  |++++++++++++++++                                  | 32% ~02m 15s      
  |+++++++++++++++++                                 | 33% ~02m 13s      
  |+++++++++++++++++                                 | 34% ~02m 11s      
  |++++++++++++++++++                                | 35% ~02m 09s      
  |++++++++++++++++++                                | 36% ~02m 07s      
  |+++++++++++++++++++                               | 37% ~02m 05s      
  |+++++++++++++++++++                               | 38% ~02m 03s      
  |++++++++++++++++++++                              | 39% ~02m 01s      
  |++++++++++++++++++++                              | 40% ~01m 59s      
  |+++++++++++++++++++++                             | 41% ~01m 57s      
  |+++++++++++++++++++++                             | 42% ~01m 55s      
  |++++++++++++++++++++++                            | 43% ~01m 53s      
  |++++++++++++++++++++++                            | 44% ~01m 51s      
  |+++++++++++++++++++++++                           | 45% ~01m 49s      
  |+++++++++++++++++++++++                           | 46% ~01m 47s      
  |++++++++++++++++++++++++                          | 47% ~01m 45s      
  |++++++++++++++++++++++++                          | 48% ~01m 43s      
  |+++++++++++++++++++++++++                         | 49% ~01m 41s      
  |+++++++++++++++++++++++++                         | 50% ~01m 39s      
  |++++++++++++++++++++++++++                        | 51% ~01m 37s      
  |+++++++++++++++++++++++++++                       | 52% ~01m 35s      
  |+++++++++++++++++++++++++++                       | 53% ~01m 33s      
  |++++++++++++++++++++++++++++                      | 54% ~01m 31s      
  |++++++++++++++++++++++++++++                      | 55% ~01m 29s      
  |+++++++++++++++++++++++++++++                     | 56% ~01m 27s      
  |+++++++++++++++++++++++++++++                     | 57% ~01m 25s      
  |++++++++++++++++++++++++++++++                    | 58% ~01m 23s      
  |++++++++++++++++++++++++++++++                    | 59% ~01m 21s      
  |+++++++++++++++++++++++++++++++                   | 60% ~01m 19s      
  |+++++++++++++++++++++++++++++++                   | 61% ~01m 17s      
  |++++++++++++++++++++++++++++++++                  | 62% ~01m 15s      
  |++++++++++++++++++++++++++++++++                  | 63% ~01m 13s      
  |+++++++++++++++++++++++++++++++++                 | 64% ~01m 11s      
  |+++++++++++++++++++++++++++++++++                 | 65% ~01m 09s      
  |++++++++++++++++++++++++++++++++++                | 66% ~01m 07s      
  |++++++++++++++++++++++++++++++++++                | 67% ~01m 05s      
  |+++++++++++++++++++++++++++++++++++               | 68% ~01m 03s      
  |+++++++++++++++++++++++++++++++++++               | 69% ~01m 01s      
  |++++++++++++++++++++++++++++++++++++              | 70% ~59s          
  |++++++++++++++++++++++++++++++++++++              | 71% ~57s          
  |+++++++++++++++++++++++++++++++++++++             | 72% ~55s          
  |+++++++++++++++++++++++++++++++++++++             | 73% ~53s          
  |++++++++++++++++++++++++++++++++++++++            | 74% ~51s          
  |++++++++++++++++++++++++++++++++++++++            | 76% ~49s          
  |+++++++++++++++++++++++++++++++++++++++           | 77% ~47s          
  |+++++++++++++++++++++++++++++++++++++++           | 78% ~45s          
  |++++++++++++++++++++++++++++++++++++++++          | 79% ~43s          
  |++++++++++++++++++++++++++++++++++++++++          | 80% ~41s          
  |+++++++++++++++++++++++++++++++++++++++++         | 81% ~39s          
  |+++++++++++++++++++++++++++++++++++++++++         | 82% ~37s          
  |++++++++++++++++++++++++++++++++++++++++++        | 83% ~35s          
  |++++++++++++++++++++++++++++++++++++++++++        | 84% ~33s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~31s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 86% ~29s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~27s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 88% ~25s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~23s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 90% ~21s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~18s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 92% ~16s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~14s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 94% ~12s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~10s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~08s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~06s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~04s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~02s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=03m 21s
Calculating cluster Periportal LSEC

  |                                                  | 0 % ~calculating  
  |+                                                 | 1 % ~01m 34s      
  |++                                                | 2 % ~01m 33s      
  |++                                                | 3 % ~01m 33s      
  |+++                                               | 4 % ~01m 32s      
  |+++                                               | 5 % ~01m 31s      
  |++++                                              | 6 % ~01m 30s      
  |++++                                              | 7 % ~01m 29s      
  |+++++                                             | 8 % ~01m 28s      
  |+++++                                             | 9 % ~01m 27s      
  |++++++                                            | 10% ~01m 26s      
  |++++++                                            | 11% ~01m 25s      
  |+++++++                                           | 12% ~01m 23s      
  |+++++++                                           | 13% ~01m 22s      
  |++++++++                                          | 14% ~01m 22s      
  |++++++++                                          | 15% ~01m 21s      
  |+++++++++                                         | 16% ~01m 20s      
  |+++++++++                                         | 18% ~01m 19s      
  |++++++++++                                        | 19% ~01m 18s      
  |++++++++++                                        | 20% ~01m 17s      
  |+++++++++++                                       | 21% ~01m 16s      
  |+++++++++++                                       | 22% ~01m 15s      
  |++++++++++++                                      | 23% ~01m 14s      
  |++++++++++++                                      | 24% ~01m 13s      
  |+++++++++++++                                     | 25% ~01m 12s      
  |+++++++++++++                                     | 26% ~01m 11s      
  |++++++++++++++                                    | 27% ~01m 10s      
  |++++++++++++++                                    | 28% ~01m 09s      
  |+++++++++++++++                                   | 29% ~01m 08s      
  |+++++++++++++++                                   | 30% ~01m 07s      
  |++++++++++++++++                                  | 31% ~01m 06s      
  |++++++++++++++++                                  | 32% ~01m 05s      
  |+++++++++++++++++                                 | 33% ~01m 04s      
  |++++++++++++++++++                                | 34% ~01m 03s      
  |++++++++++++++++++                                | 35% ~01m 02s      
  |+++++++++++++++++++                               | 36% ~01m 01s      
  |+++++++++++++++++++                               | 37% ~01m 00s      
  |++++++++++++++++++++                              | 38% ~59s          
  |++++++++++++++++++++                              | 39% ~58s          
  |+++++++++++++++++++++                             | 40% ~57s          
  |+++++++++++++++++++++                             | 41% ~56s          
  |++++++++++++++++++++++                            | 42% ~55s          
  |++++++++++++++++++++++                            | 43% ~54s          
  |+++++++++++++++++++++++                           | 44% ~53s          
  |+++++++++++++++++++++++                           | 45% ~52s          
  |++++++++++++++++++++++++                          | 46% ~51s          
  |++++++++++++++++++++++++                          | 47% ~50s          
  |+++++++++++++++++++++++++                         | 48% ~49s          
  |+++++++++++++++++++++++++                         | 49% ~48s          
  |++++++++++++++++++++++++++                        | 51% ~47s          
  |++++++++++++++++++++++++++                        | 52% ~46s          
  |+++++++++++++++++++++++++++                       | 53% ~45s          
  |+++++++++++++++++++++++++++                       | 54% ~44s          
  |++++++++++++++++++++++++++++                      | 55% ~43s          
  |++++++++++++++++++++++++++++                      | 56% ~42s          
  |+++++++++++++++++++++++++++++                     | 57% ~41s          
  |+++++++++++++++++++++++++++++                     | 58% ~40s          
  |++++++++++++++++++++++++++++++                    | 59% ~39s          
  |++++++++++++++++++++++++++++++                    | 60% ~38s          
  |+++++++++++++++++++++++++++++++                   | 61% ~37s          
  |+++++++++++++++++++++++++++++++                   | 62% ~36s          
  |++++++++++++++++++++++++++++++++                  | 63% ~36s          
  |++++++++++++++++++++++++++++++++                  | 64% ~35s          
  |+++++++++++++++++++++++++++++++++                 | 65% ~34s          
  |+++++++++++++++++++++++++++++++++                 | 66% ~33s          
  |++++++++++++++++++++++++++++++++++                | 67% ~32s          
  |+++++++++++++++++++++++++++++++++++               | 68% ~31s          
  |+++++++++++++++++++++++++++++++++++               | 69% ~30s          
  |++++++++++++++++++++++++++++++++++++              | 70% ~29s          
  |++++++++++++++++++++++++++++++++++++              | 71% ~28s          
  |+++++++++++++++++++++++++++++++++++++             | 72% ~27s          
  |+++++++++++++++++++++++++++++++++++++             | 73% ~26s          
  |++++++++++++++++++++++++++++++++++++++            | 74% ~25s          
  |++++++++++++++++++++++++++++++++++++++            | 75% ~24s          
  |+++++++++++++++++++++++++++++++++++++++           | 76% ~23s          
  |+++++++++++++++++++++++++++++++++++++++           | 77% ~22s          
  |++++++++++++++++++++++++++++++++++++++++          | 78% ~21s          
  |++++++++++++++++++++++++++++++++++++++++          | 79% ~20s          
  |+++++++++++++++++++++++++++++++++++++++++         | 80% ~19s          
  |+++++++++++++++++++++++++++++++++++++++++         | 81% ~18s          
  |++++++++++++++++++++++++++++++++++++++++++        | 82% ~17s          
  |++++++++++++++++++++++++++++++++++++++++++        | 84% ~16s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~15s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 86% ~14s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~13s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 88% ~12s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~11s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 90% ~10s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~09s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 92% ~08s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~07s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 94% ~06s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~05s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~04s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~03s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~02s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=01m 35s
Calculating cluster LSEC (fenestr.)

  |                                                  | 0 % ~calculating  
  |+                                                 | 1 % ~01m 60s      
  |++                                                | 2 % ~01m 60s      
  |++                                                | 3 % ~01m 59s      
  |+++                                               | 4 % ~01m 58s      
  |+++                                               | 5 % ~01m 56s      
  |++++                                              | 6 % ~01m 55s      
  |++++                                              | 7 % ~01m 53s      
  |+++++                                             | 8 % ~01m 52s      
  |+++++                                             | 9 % ~01m 51s      
  |++++++                                            | 10% ~01m 50s      
  |++++++                                            | 11% ~01m 48s      
  |+++++++                                           | 12% ~01m 49s      
  |+++++++                                           | 13% ~01m 47s      
  |++++++++                                          | 14% ~01m 46s      
  |++++++++                                          | 15% ~01m 45s      
  |+++++++++                                         | 16% ~01m 44s      
  |+++++++++                                         | 17% ~01m 42s      
  |++++++++++                                        | 18% ~01m 41s      
  |++++++++++                                        | 19% ~01m 40s      
  |+++++++++++                                       | 20% ~01m 38s      
  |+++++++++++                                       | 21% ~01m 37s      
  |++++++++++++                                      | 22% ~01m 36s      
  |++++++++++++                                      | 23% ~01m 35s      
  |+++++++++++++                                     | 24% ~01m 33s      
  |+++++++++++++                                     | 26% ~01m 32s      
  |++++++++++++++                                    | 27% ~01m 31s      
  |++++++++++++++                                    | 28% ~01m 29s      
  |+++++++++++++++                                   | 29% ~01m 28s      
  |+++++++++++++++                                   | 30% ~01m 27s      
  |++++++++++++++++                                  | 31% ~01m 26s      
  |++++++++++++++++                                  | 32% ~01m 24s      
  |+++++++++++++++++                                 | 33% ~01m 23s      
  |+++++++++++++++++                                 | 34% ~01m 22s      
  |++++++++++++++++++                                | 35% ~01m 21s      
  |++++++++++++++++++                                | 36% ~01m 19s      
  |+++++++++++++++++++                               | 37% ~01m 18s      
  |+++++++++++++++++++                               | 38% ~01m 17s      
  |++++++++++++++++++++                              | 39% ~01m 15s      
  |++++++++++++++++++++                              | 40% ~01m 14s      
  |+++++++++++++++++++++                             | 41% ~01m 13s      
  |+++++++++++++++++++++                             | 42% ~01m 12s      
  |++++++++++++++++++++++                            | 43% ~01m 10s      
  |++++++++++++++++++++++                            | 44% ~01m 09s      
  |+++++++++++++++++++++++                           | 45% ~01m 08s      
  |+++++++++++++++++++++++                           | 46% ~01m 07s      
  |++++++++++++++++++++++++                          | 47% ~01m 05s      
  |++++++++++++++++++++++++                          | 48% ~01m 04s      
  |+++++++++++++++++++++++++                         | 49% ~01m 03s      
  |+++++++++++++++++++++++++                         | 50% ~01m 02s      
  |++++++++++++++++++++++++++                        | 51% ~01m 00s      
  |+++++++++++++++++++++++++++                       | 52% ~59s          
  |+++++++++++++++++++++++++++                       | 53% ~58s          
  |++++++++++++++++++++++++++++                      | 54% ~57s          
  |++++++++++++++++++++++++++++                      | 55% ~56s          
  |+++++++++++++++++++++++++++++                     | 56% ~54s          
  |+++++++++++++++++++++++++++++                     | 57% ~53s          
  |++++++++++++++++++++++++++++++                    | 58% ~52s          
  |++++++++++++++++++++++++++++++                    | 59% ~50s          
  |+++++++++++++++++++++++++++++++                   | 60% ~49s          
  |+++++++++++++++++++++++++++++++                   | 61% ~48s          
  |++++++++++++++++++++++++++++++++                  | 62% ~47s          
  |++++++++++++++++++++++++++++++++                  | 63% ~45s          
  |+++++++++++++++++++++++++++++++++                 | 64% ~44s          
  |+++++++++++++++++++++++++++++++++                 | 65% ~43s          
  |++++++++++++++++++++++++++++++++++                | 66% ~42s          
  |++++++++++++++++++++++++++++++++++                | 67% ~40s          
  |+++++++++++++++++++++++++++++++++++               | 68% ~39s          
  |+++++++++++++++++++++++++++++++++++               | 69% ~38s          
  |++++++++++++++++++++++++++++++++++++              | 70% ~36s          
  |++++++++++++++++++++++++++++++++++++              | 71% ~35s          
  |+++++++++++++++++++++++++++++++++++++             | 72% ~34s          
  |+++++++++++++++++++++++++++++++++++++             | 73% ~33s          
  |++++++++++++++++++++++++++++++++++++++            | 74% ~31s          
  |++++++++++++++++++++++++++++++++++++++            | 76% ~30s          
  |+++++++++++++++++++++++++++++++++++++++           | 77% ~29s          
  |+++++++++++++++++++++++++++++++++++++++           | 78% ~28s          
  |++++++++++++++++++++++++++++++++++++++++          | 79% ~26s          
  |++++++++++++++++++++++++++++++++++++++++          | 80% ~25s          
  |+++++++++++++++++++++++++++++++++++++++++         | 81% ~24s          
  |+++++++++++++++++++++++++++++++++++++++++         | 82% ~23s          
  |++++++++++++++++++++++++++++++++++++++++++        | 83% ~21s          
  |++++++++++++++++++++++++++++++++++++++++++        | 84% ~20s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~19s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 86% ~18s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~16s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 88% ~15s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~14s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 90% ~13s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~11s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 92% ~10s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~09s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 94% ~08s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~06s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~05s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~04s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~03s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=02m 03s
Calculating cluster Lymphatic EC

  |                                                  | 0 % ~calculating  
  |+                                                 | 1 % ~02m 14s      
  |+                                                 | 2 % ~02m 13s      
  |++                                                | 3 % ~02m 13s      
  |++                                                | 4 % ~02m 11s      
  |+++                                               | 5 % ~02m 10s      
  |+++                                               | 6 % ~02m 09s      
  |++++                                              | 7 % ~02m 11s      
  |++++                                              | 8 % ~02m 10s      
  |+++++                                             | 9 % ~02m 08s      
  |+++++                                             | 10% ~02m 06s      
  |++++++                                            | 11% ~02m 05s      
  |++++++                                            | 12% ~02m 11s      
  |+++++++                                           | 13% ~02m 08s      
  |+++++++                                           | 14% ~02m 06s      
  |++++++++                                          | 15% ~02m 04s      
  |++++++++                                          | 16% ~02m 02s      
  |+++++++++                                         | 17% ~02m 00s      
  |+++++++++                                         | 18% ~01m 59s      
  |++++++++++                                        | 19% ~01m 57s      
  |++++++++++                                        | 20% ~01m 55s      
  |+++++++++++                                       | 21% ~01m 54s      
  |+++++++++++                                       | 22% ~01m 52s      
  |++++++++++++                                      | 23% ~01m 50s      
  |++++++++++++                                      | 24% ~01m 49s      
  |+++++++++++++                                     | 25% ~01m 47s      
  |+++++++++++++                                     | 26% ~01m 45s      
  |++++++++++++++                                    | 27% ~01m 44s      
  |++++++++++++++                                    | 28% ~01m 42s      
  |+++++++++++++++                                   | 29% ~01m 41s      
  |+++++++++++++++                                   | 30% ~01m 39s      
  |++++++++++++++++                                  | 31% ~01m 38s      
  |++++++++++++++++                                  | 32% ~01m 36s      
  |+++++++++++++++++                                 | 33% ~01m 35s      
  |+++++++++++++++++                                 | 34% ~01m 33s      
  |++++++++++++++++++                                | 35% ~01m 32s      
  |++++++++++++++++++                                | 36% ~01m 30s      
  |+++++++++++++++++++                               | 37% ~01m 29s      
  |+++++++++++++++++++                               | 38% ~01m 27s      
  |++++++++++++++++++++                              | 39% ~01m 26s      
  |++++++++++++++++++++                              | 40% ~01m 25s      
  |+++++++++++++++++++++                             | 41% ~01m 24s      
  |+++++++++++++++++++++                             | 42% ~01m 23s      
  |++++++++++++++++++++++                            | 43% ~01m 22s      
  |++++++++++++++++++++++                            | 44% ~01m 21s      
  |+++++++++++++++++++++++                           | 45% ~01m 19s      
  |+++++++++++++++++++++++                           | 46% ~01m 18s      
  |++++++++++++++++++++++++                          | 47% ~01m 16s      
  |++++++++++++++++++++++++                          | 48% ~01m 15s      
  |+++++++++++++++++++++++++                         | 49% ~01m 13s      
  |+++++++++++++++++++++++++                         | 50% ~01m 12s      
  |++++++++++++++++++++++++++                        | 51% ~01m 10s      
  |++++++++++++++++++++++++++                        | 52% ~01m 09s      
  |+++++++++++++++++++++++++++                       | 53% ~01m 07s      
  |+++++++++++++++++++++++++++                       | 54% ~01m 06s      
  |++++++++++++++++++++++++++++                      | 55% ~01m 04s      
  |++++++++++++++++++++++++++++                      | 56% ~01m 03s      
  |+++++++++++++++++++++++++++++                     | 57% ~01m 01s      
  |+++++++++++++++++++++++++++++                     | 58% ~60s          
  |++++++++++++++++++++++++++++++                    | 59% ~58s          
  |++++++++++++++++++++++++++++++                    | 60% ~57s          
  |+++++++++++++++++++++++++++++++                   | 61% ~55s          
  |+++++++++++++++++++++++++++++++                   | 62% ~54s          
  |++++++++++++++++++++++++++++++++                  | 63% ~53s          
  |++++++++++++++++++++++++++++++++                  | 64% ~51s          
  |+++++++++++++++++++++++++++++++++                 | 65% ~50s          
  |+++++++++++++++++++++++++++++++++                 | 66% ~48s          
  |++++++++++++++++++++++++++++++++++                | 67% ~47s          
  |++++++++++++++++++++++++++++++++++                | 68% ~45s          
  |+++++++++++++++++++++++++++++++++++               | 69% ~44s          
  |+++++++++++++++++++++++++++++++++++               | 70% ~42s          
  |++++++++++++++++++++++++++++++++++++              | 71% ~41s          
  |++++++++++++++++++++++++++++++++++++              | 72% ~40s          
  |+++++++++++++++++++++++++++++++++++++             | 73% ~38s          
  |+++++++++++++++++++++++++++++++++++++             | 74% ~37s          
  |++++++++++++++++++++++++++++++++++++++            | 75% ~35s          
  |++++++++++++++++++++++++++++++++++++++            | 76% ~34s          
  |+++++++++++++++++++++++++++++++++++++++           | 77% ~32s          
  |+++++++++++++++++++++++++++++++++++++++           | 78% ~31s          
  |++++++++++++++++++++++++++++++++++++++++          | 79% ~30s          
  |++++++++++++++++++++++++++++++++++++++++          | 80% ~28s          
  |+++++++++++++++++++++++++++++++++++++++++         | 81% ~27s          
  |+++++++++++++++++++++++++++++++++++++++++         | 82% ~25s          
  |++++++++++++++++++++++++++++++++++++++++++        | 83% ~24s          
  |++++++++++++++++++++++++++++++++++++++++++        | 84% ~23s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~21s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 86% ~20s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~18s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 88% ~17s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~15s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 90% ~14s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~13s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 92% ~11s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~10s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 94% ~08s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~07s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~06s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~04s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~03s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=02m 19s
Calculating cluster Cycling cells

  |                                                  | 0 % ~calculating  
  |+                                                 | 1 % ~02m 53s      
  |+                                                 | 2 % ~02m 52s      
  |++                                                | 3 % ~02m 50s      
  |++                                                | 4 % ~02m 48s      
  |+++                                               | 5 % ~02m 47s      
  |+++                                               | 6 % ~02m 45s      
  |++++                                              | 7 % ~02m 43s      
  |++++                                              | 8 % ~02m 41s      
  |+++++                                             | 9 % ~02m 39s      
  |+++++                                             | 10% ~02m 38s      
  |++++++                                            | 11% ~02m 36s      
  |++++++                                            | 12% ~02m 34s      
  |+++++++                                           | 13% ~02m 33s      
  |+++++++                                           | 14% ~02m 31s      
  |++++++++                                          | 15% ~02m 29s      
  |++++++++                                          | 16% ~02m 27s      
  |+++++++++                                         | 17% ~02m 26s      
  |+++++++++                                         | 18% ~02m 24s      
  |++++++++++                                        | 19% ~02m 22s      
  |++++++++++                                        | 20% ~02m 20s      
  |+++++++++++                                       | 21% ~02m 19s      
  |+++++++++++                                       | 22% ~02m 17s      
  |++++++++++++                                      | 23% ~02m 15s      
  |++++++++++++                                      | 24% ~02m 13s      
  |+++++++++++++                                     | 25% ~02m 12s      
  |+++++++++++++                                     | 26% ~02m 10s      
  |++++++++++++++                                    | 27% ~02m 08s      
  |++++++++++++++                                    | 28% ~02m 06s      
  |+++++++++++++++                                   | 29% ~02m 05s      
  |+++++++++++++++                                   | 30% ~02m 03s      
  |++++++++++++++++                                  | 31% ~02m 01s      
  |++++++++++++++++                                  | 32% ~01m 59s      
  |+++++++++++++++++                                 | 33% ~01m 58s      
  |+++++++++++++++++                                 | 34% ~01m 56s      
  |++++++++++++++++++                                | 35% ~01m 54s      
  |++++++++++++++++++                                | 36% ~01m 52s      
  |+++++++++++++++++++                               | 37% ~01m 51s      
  |+++++++++++++++++++                               | 38% ~01m 49s      
  |++++++++++++++++++++                              | 39% ~01m 47s      
  |++++++++++++++++++++                              | 40% ~01m 45s      
  |+++++++++++++++++++++                             | 41% ~01m 44s      
  |+++++++++++++++++++++                             | 42% ~01m 42s      
  |++++++++++++++++++++++                            | 43% ~01m 40s      
  |++++++++++++++++++++++                            | 44% ~01m 38s      
  |+++++++++++++++++++++++                           | 45% ~01m 37s      
  |+++++++++++++++++++++++                           | 46% ~01m 35s      
  |++++++++++++++++++++++++                          | 47% ~01m 33s      
  |++++++++++++++++++++++++                          | 48% ~01m 31s      
  |+++++++++++++++++++++++++                         | 49% ~01m 29s      
  |+++++++++++++++++++++++++                         | 50% ~01m 28s      
  |++++++++++++++++++++++++++                        | 51% ~01m 26s      
  |++++++++++++++++++++++++++                        | 52% ~01m 24s      
  |+++++++++++++++++++++++++++                       | 53% ~01m 23s      
  |+++++++++++++++++++++++++++                       | 54% ~01m 21s      
  |++++++++++++++++++++++++++++                      | 55% ~01m 19s      
  |++++++++++++++++++++++++++++                      | 56% ~01m 17s      
  |+++++++++++++++++++++++++++++                     | 57% ~01m 15s      
  |+++++++++++++++++++++++++++++                     | 58% ~01m 14s      
  |++++++++++++++++++++++++++++++                    | 59% ~01m 12s      
  |++++++++++++++++++++++++++++++                    | 60% ~01m 10s      
  |+++++++++++++++++++++++++++++++                   | 61% ~01m 08s      
  |+++++++++++++++++++++++++++++++                   | 62% ~01m 07s      
  |++++++++++++++++++++++++++++++++                  | 63% ~01m 05s      
  |++++++++++++++++++++++++++++++++                  | 64% ~01m 03s      
  |+++++++++++++++++++++++++++++++++                 | 65% ~01m 01s      
  |+++++++++++++++++++++++++++++++++                 | 66% ~60s          
  |++++++++++++++++++++++++++++++++++                | 67% ~58s          
  |++++++++++++++++++++++++++++++++++                | 68% ~56s          
  |+++++++++++++++++++++++++++++++++++               | 69% ~54s          
  |+++++++++++++++++++++++++++++++++++               | 70% ~53s          
  |++++++++++++++++++++++++++++++++++++              | 71% ~51s          
  |++++++++++++++++++++++++++++++++++++              | 72% ~49s          
  |+++++++++++++++++++++++++++++++++++++             | 73% ~47s          
  |+++++++++++++++++++++++++++++++++++++             | 74% ~46s          
  |++++++++++++++++++++++++++++++++++++++            | 75% ~44s          
  |++++++++++++++++++++++++++++++++++++++            | 76% ~42s          
  |+++++++++++++++++++++++++++++++++++++++           | 77% ~41s          
  |+++++++++++++++++++++++++++++++++++++++           | 78% ~39s          
  |++++++++++++++++++++++++++++++++++++++++          | 79% ~37s          
  |++++++++++++++++++++++++++++++++++++++++          | 80% ~35s          
  |+++++++++++++++++++++++++++++++++++++++++         | 81% ~33s          
  |+++++++++++++++++++++++++++++++++++++++++         | 82% ~32s          
  |++++++++++++++++++++++++++++++++++++++++++        | 83% ~30s          
  |++++++++++++++++++++++++++++++++++++++++++        | 84% ~28s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~26s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 86% ~25s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~23s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 88% ~21s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~19s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 90% ~18s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~16s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 92% ~14s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~12s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 94% ~11s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~09s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~07s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~05s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~04s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~02s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=02m 55s
write.csv(mkend_simp[mkend_simp$p_val_adj<=0.05,], 
          file = "results/endothelial/markers_endo_subpop_simp.csv", row.names = T, quote = F)

Normalise, PCA, UMAP

lsec_list = list(lsec_end_cells = only_end_cells[,only_end_cells$pca25_res.0.9 %in% c(0, 1, 3, 12)])

Get DPT

h_lsec_list = list()
for(n in names(lsec_list)){
  h_lsec_list[[n]] = lsec_list[[n]][,lsec_list[[n]]$Condition=="healthy"]
  h_lsec_list[[n]] = suppressWarnings(SCTransform(h_lsec_list[[n]], do.correct.umi = T, verbose = F, 
                                                  vars.to.regress=c("unique_name","nCount_RNA"),
                                                  variable.features.rv.th = 1, seed.use = 1,
                                                  return.only.var.genes = F, 
                                                  variable.features.n = NULL))
  h_lsec_list[[n]] = RunPCA(h_lsec_list[[n]], verbose = F)
  h_lsec_list[[n]] = RunUMAP(h_lsec_list[[n]], dims = 1:20, verbose = F)
}

Plot DPT as violin

We’re using subset 2 - only bona fide LSEC

for(n in names(h_lsec_list)){
  plot_df = data.frame(row.names = rownames(dpt_l[[n]]@branch), 
                       "cl" = h_lsec_list[[n]]$endo_simp,
                       "dpt" = getDPT(dpt_l[[n]]))
  
  plt = ggplot(plot_df, aes(y = cl, x = dpt, fill = cl))+
    geom_boxplot()+
    labs(title = n)+
    theme_bw()+
    theme(axis.text.x = element_text(size = 7.5))
  print(plt)
}

Plot pseudotime as boxplot

Find genes varying along the pseudotime

plot_df = data.frame(row.names = rownames(dpt@branch), 
                     "cl" = sub_2$endo_simp,
                     "dpt" = getDPT(dpt))

plt = ggplot(plot_df, aes(y = cl, x = rank(dpt), fill = cl))+
  geom_boxplot()+
  theme_bw()+
  theme(axis.text.x = element_text(size = 7.5))
print(plt)

Now use the top genes to learn the pseudozonation coordinates and project the embolised and regenerating data

df_dc = cbind(sub_2@meta.data, 
              data.frame("DC1" = dpt@dm$DC1, "DC2" = dpt@dm$DC2, 
                         "DPT" = scales::rescale(rank(getDPT(dpt)), c(0.00001, 0.99999))))

# will only use variable genes
hvg = sub_2@assays$SCT@var.features

# Fit GAM for each gene using pseudotime as independent variable.
t = df_dc$DPT
gene_fit_p = c()
gene_fit_vals = data.frame(row.names = 1:100)
for(i in 1:length(hvg)){
  g = hvg[i]
  z = sub_2@assays$SCT@data[g,]
  
  d = data.frame(z=z, t=t)
  tmp = suppressMessages(gam(z ~ ns(t, df = 3), data=d))
  
  # bins for model fitting
  bb = seq(min(t), max(t), length.out = 100)
  gene_fit_vals[,g] = suppressMessages(predict(tmp,
                                               newdata = data.frame(t = bb)))
  p = summary(tmp)$parametric.anova$`Pr(>F)`[1]
  gene_fit_p = c(gene_fit_p, p)
  names(gene_fit_p)[length(gene_fit_p)] = g
}

gene_fit_p = fdrtool::fdrtool(gene_fit_p, statistic="pvalue", plot=F, 
                              verbose=F, cutoff.method="pct0", pct0=0.9)$qval
sig_genes = gene_fit_p[gene_fit_p<=0.05]
top_sig_genes = names(sig_genes[order(sig_genes, decreasing = F)][1:1000])
top_sig_genes = top_sig_genes[top_sig_genes %in% rownames(only_end_cells@assays$SCT@data)]

data_gam_h = cbind(data.frame("DPT" = df_dc$DPT),
                   Matrix::t(sub_2@assays$SCT@data[top_sig_genes,]))

gam_healthy = gam::gam(DPT ~ ., data = data_gam_h, 
                       family = mgcv::betar(link = "logit", eps = 0.00001))

all_pred = predict(gam_healthy,
                   Matrix::t(only_end_cells@assays$SCT@data[top_sig_genes,]), type= "response")

goodcl = only_end_cells$pca25_res.0.9 %in% sub_2$pca25_res.0.9
all_pred_sub = all_pred[goodcl & only_end_cells$Condition!="healthy" | names(all_pred) %in% colnames(sub_2)]

traj_list = list("healthy" = data_gam_h$DPT, 
                 "embolised" = all_pred[goodcl & only_end_cells$Condition!="embolised"], 
                 "regenerating" = all_pred[goodcl & only_end_cells$Condition!="regenerating"])

only_end_cells = AddMetaData(only_end_cells, 
                             data.frame("zonation_pt" = all_pred, 
                                        "goodcl" = colnames(only_end_cells) %in% names(all_pred_sub)))
only_end_cells@meta.data[rownames(data_gam_h),"zonation_pt"] = data_gam_h$DPT

plot_df = only_end_cells@meta.data[names(all_pred_sub),]
plot_df$bins100 = cut(plot_df$zonation_pt, 10)
plot_df$Condition = factor(plot_df$Condition, levels = c("healthy", "embolised", "regenerating"))
ggplot(plot_df, aes(x = bins100, fill = endo_simp))+
     facet_wrap(~Condition)+
     geom_bar()+
     labs(x = "zonation (binned)", y = "Number of cells")+
     scale_y_continuous(expand = c(0,0))+
     coord_flip()+
     guides(fill = guide_legend(title.position = "top"))+
  theme_classic()+
     theme(axis.text.y = element_blank(),
           strip.background = element_rect(fill = colcond),
           legend.title.align = 0,
           legend.position = "bottom")

VlnPlot(only_end_cells[,names(all_pred_sub)], features = "nCount_RNA", 
        group.by = "endo_simp", split.by = "Condition")

ggplot(plot_df, aes(x = zonation_pt, y = endo_simp, fill = endo_simp))+
     facet_wrap(~Condition)+
     geom_violin()+
     labs(x = "zonation", y = "clusters")+
     scale_x_continuous(expand = c(0,0))+
     guides(fill = guide_legend(title.position = "top"))+
     theme_bw()+
     theme(axis.text.y = element_blank(),
           legend.title.align = 0,
           legend.position = "bottom")

Do cross validation on the healthy trajectory

plot_df = only_end_cells@meta.data
plot_df$pt = all_pred[colnames(only_end_cells)]
plot_df$bins100 = cut(plot_df$pt, 10)
plot_df$Condition = factor(plot_df$Condition, levels = c("healthy", "embolised", "regenerating"))

ggplot(plot_df, aes(x = pt, y = endo_simp, fill = endo_simp))+
     facet_wrap(~Condition)+
     geom_violin()+
     labs(x = "zonation", y = "clusters")+
     scale_x_continuous(expand = c(0,0))+
     guides(fill = guide_legend(title.position = "top", reverse = T))+
     theme_bw()+
     theme(axis.text.y = element_blank(),
           legend.title.align = 0,
           legend.position = "right")

Find varying genes - using all cells along the ranked pseudotime

sig_genes = gene_fit_p[gene_fit_p<=0.05]
top_sig_genes = names(sig_genes[order(sig_genes, decreasing = F)][1:1000]) 

data_gam_h = cbind(data.frame("DPT" = traj_list$healthy),
                   Matrix::t(sub_2@assays$SCT@data[top_sig_genes,]))
colnames(data_gam_h) = gsub("-", "_", colnames(data_gam_h), fixed = T)
colnames(data_gam_h) = gsub(".", "_", colnames(data_gam_h), fixed = T)

cv_gam = CVgam(data = data_gam_h, formula = DPT ~ ., nfold = 10, seed = 1, method = "glm.fit")
CV-mse-GAM  
      0.044 
pdf("results/endothelial/end_original_fitted_pseudotime.pdf", useDingbats = F, 
    width = 6, height = 5)
plot(data_gam_h$DPT, cv_gam$fitted, xlab = "Healthy pseudotime",ylab = "Predicted Healthy",
     main = paste0("Healthy original vs fitted pseudotime\nMSE: ", round(cv_gam$cvscale, 6)),
     pch = 19, cex = 0.5)
abline(0,1, col = "blue", lwd = 3)
dev.off()
null device 
          1 
plot_df = data.frame("original" = data_gam_h$DPT, "predicted" = cv_gam$fitted)
plot_df$cl = sub_2@meta.data$endo_simp
ggplot(plot_df, aes(x = original, y = predicted, colour = cl))+geom_point()

Correlate genes between conditions

## get varying genes for each condition
numCores = 12 #larger values resulted in problems
start_time <- Sys.time()
cond_fit_list = list()

only_end_cells_bf = only_end_cells[,only_end_cells$pca25_res.0.9 %in% sub_2$pca25_res.0.9]
only_end_cells_split = SplitObject(only_end_cells_bf, split.by = "Condition")

for(cond in names(only_end_cells_split)){
  print(cond)
  
  sub_dat = only_end_cells_split[[cond]]
  # Fit GAM for each gene using pseudotime as independent variable.
  cond_fit_list[[cond]] = mclapply(hvg, fittingFunc, mc.cores = numCores)
}
[1] "healthy"
[1] "embolised"
[1] "regenerating"
end_fits_qval = list()
for(cond in names(cond_fit_list)){
  fits_df = Reduce(cbind, lapply(cond_fit_list[[cond]], function(x) x$fits))
  
  pval_list = unlist(lapply(cond_fit_list[[cond]], function(x) x$pval))
  names(pval_list) = hvg
  
  pval_df = data.frame(pval_list)
  
  end_fits_qval[[cond]] = list("fits" = fits_df, "pval" = pval_df,
                               "fdr" = apply(pval_df, 2, function(x) p.adjust(x, method = "fdr")))
}
end_time <- Sys.time()
end_time - start_time
Time difference of 2.806205 mins
saveRDS(end_fits_qval, file = "results/endothelial/end_fits_qval.RDS")

GO Term enrichment

g = "LYVE1"
plot(end_fits_qval$healthy$fits[,g])
points(end_fits_qval$embolised$fits[,g], col = "red")
points(end_fits_qval$regenerating$fits[,g], col = "blue")


all_var_genes = (end_fits_qval$healthy$fdr<=0.05 & 
  apply(end_fits_qval$healthy$fits, 2, function(x) diff(range(x))>=0.05)) | 
  (end_fits_qval$embolised$fdr<=0.05 & 
  apply(end_fits_qval$embolised$fits, 2, function(x) diff(range(x))>=0.05)) | 
  (end_fits_qval$regenerating$fdr<=0.05 & 
  apply(end_fits_qval$regenerating$fits, 2, function(x) diff(range(x))>=0.05))

end_cor_he = sapply(rownames(all_var_genes)[all_var_genes], 
                    function(g) cor(end_fits_qval$healthy$fits[,g],end_fits_qval$embolised$fits[,g], 
                                    method = "sp"), simplify = T)
end_cor_hr = sapply(rownames(all_var_genes)[all_var_genes], 
                    function(g) cor(end_fits_qval$healthy$fits[,g],end_fits_qval$regenerating$fits[,g], 
                                    method = "sp"), simplify = T)
end_cor_er = sapply(rownames(all_var_genes)[all_var_genes], 
                    function(g) cor(end_fits_qval$embolised$fits[,g],end_fits_qval$regenerating$fits[,g], 
                                    method = "sp"), simplify = T)

cor_g_df = data.frame("gene" = names(end_cor_he), 
                      "HvE" = end_cor_he, "HvR" = end_cor_hr, "EvR" = end_cor_er)
write.csv(cor_g_df, file = "results/endothelial/correlations_zonation_endothelial.csv", 
          row.names = T, quote = F)

Plot top genes in correlation

got_cor = list()
eg_all = clusterProfiler::bitr(colnames(end_fits_qval$healthy$fits), fromType="SYMBOL", 
                             toType="ENTREZID", OrgDb="org.Hs.eg.db")
'select()' returned 1:1 mapping between keys and columns
10.73% of input gene IDs are fail to map...
eg = clusterProfiler::bitr(names(end_cor_he)[end_cor_he<0.3], fromType="SYMBOL", 
                           toType="ENTREZID", OrgDb="org.Hs.eg.db")
'select()' returned 1:1 mapping between keys and columns
5.94% of input gene IDs are fail to map...
got = clusterProfiler::enrichGO(gene = eg$ENTREZID, universe = eg_all$ENTREZID, 
                                OrgDb = org.Hs.eg.db, ont = "BP", pvalueCutoff  = 0.01,
                                qvalueCutoff  = 0.05, 
                                pAdjustMethod = "BH", readable = T)
got_cor[["notcor_he"]] = getTopTerms(got@result[got@result$p.adjust<=0.05,], ncl = 4, nt = 2, topt = 1000)

eg = clusterProfiler::bitr(names(end_cor_he)[end_cor_he>=0.3], fromType="SYMBOL", 
                           toType="ENTREZID", OrgDb="org.Hs.eg.db")
'select()' returned 1:1 mapping between keys and columns
4.83% of input gene IDs are fail to map...
got = clusterProfiler::enrichGO(gene = eg$ENTREZID, universe = eg_all$ENTREZID, 
                                OrgDb = org.Hs.eg.db, ont = "BP", pvalueCutoff  = 0.01,
                                qvalueCutoff  = 0.05, 
                                pAdjustMethod = "BH", readable = T)
got_cor[["cor_he"]] = getTopTerms(got@result[got@result$p.adjust<=0.05,], ncl = 4, nt = 2, topt = 1000)

eg = clusterProfiler::bitr(names(end_cor_hr)[end_cor_hr<0.3], fromType="SYMBOL", 
                           toType="ENTREZID", OrgDb="org.Hs.eg.db")
'select()' returned 1:1 mapping between keys and columns
5.79% of input gene IDs are fail to map...
got = clusterProfiler::enrichGO(gene = eg$ENTREZID, universe = eg_all$ENTREZID, 
                                OrgDb = org.Hs.eg.db, ont = "BP", pvalueCutoff  = 0.01,
                                qvalueCutoff  = 0.05, 
                                pAdjustMethod = "BH", readable = T)
got_cor[["notcor_hr"]] = getTopTerms(got@result[got@result$p.adjust<=0.05,], ncl = 4, nt = 2, topt = 1000)

eg = clusterProfiler::bitr(names(end_cor_hr)[end_cor_hr>=0.3], fromType="SYMBOL", 
                           toType="ENTREZID", OrgDb="org.Hs.eg.db")
'select()' returned 1:1 mapping between keys and columns
4.9% of input gene IDs are fail to map...
got = clusterProfiler::enrichGO(gene = eg$ENTREZID, universe = eg_all$ENTREZID, 
                                OrgDb = org.Hs.eg.db, ont = "BP", pvalueCutoff  = 0.01,
                                qvalueCutoff  = 0.05, 
                                pAdjustMethod = "BH", readable = T)
got_cor[["cor_hr"]] = getTopTerms(got@result[got@result$p.adjust<=0.05,], ncl = 4, nt = 2, topt = 1000)

saveRDS(got_cor, file = "results/endothelial/GOTerms_correlations.RDS")

Save Seurat with predictions

topgenes = unique(c(names(end_cor_he[order(end_cor_he, decreasing = T)])[1:30],
             names(end_cor_hr[order(end_cor_hr, decreasing = T)])[1:30]))
bottomgenes = unique(c(names(end_cor_he[order(end_cor_he, decreasing = F)])[1:30],
             names(end_cor_hr[order(end_cor_hr, decreasing = F)])[1:30]))
usegenes = c(topgenes, bottomgenes)

bins_list = list()
for(n in names(only_end_cells_split)){
  plot_df = cbind(data.frame("pt" = rep(1:10, each = 10)),end_fits_qval[[n]]$fits[,usegenes])
  plot_df$bins10 = cut(plot_df$pt, 10)
  bins_list[[n]] = sapply(usegenes, function(x) scale(tapply(plot_df[,x], plot_df$bins10, mean))[10:1])
}
bins_mean = Reduce(rbind, bins_list)

hct = hclust(dist(t(bins_list$healthy[,topgenes])), method = "ward.D2")
ord_bot_h = apply(bins_list$healthy[,bottomgenes], 2, which.max)
ord_bot_e = apply(bins_list$embolised[,bottomgenes], 2, which.max)
ord_bot_r = apply(bins_list$regenerating[,bottomgenes], 2, which.max)
ord_df = data.frame(ord_bot_h, ord_bot_e, ord_bot_r)
ord_bot = rownames(ord_df)[with(ord_df, order(ord_bot_h, ord_bot_e, ord_bot_r, decreasing = T))]
ord = c(topgenes[hct$order], ord_bot)

pheatmap::pheatmap(bins_mean[,ord], show_rownames = F, fontsize_col = 6.5, border_color = NA,
                   gaps_row = c(10,20), gaps_col = c(length(topgenes)),
                   clustering_method = "ward.D2", cluster_rows = F, cluster_cols = F)

---
title: "R Notebook"
output: html_notebook
---



# General Setup
Setup chunk

```{r, setup, include=FALSE}
knitr::opts_chunk$set(fig.width = 8)
knitr::opts_knit$set(root.dir = normalizePath(".."))
knitr::opts_knit$get("root.dir")
```

Setup reticulate

```{r}
library(reticulate)
knitr::knit_engines$set(python = reticulate::eng_python)
py_available(initialize = FALSE)
use_python(Sys.which("python"))
py_config()
```

Load libraries

```{r}
library(Seurat)
library(ggplot2)
library(destiny)
library(plyr)
library(dplyr)
library(RColorBrewer)
```

Functions

```{r}
getDPT = function(x){
  return(destiny:::dpt_for_branch(destiny::branch_divide(x, integer(0L)), 
                                  min(destiny::branch_divide(x, integer(0L))@branch[, 1], 
                                      na.rm = TRUE)))
}
CVgam = function (formula, data, nfold = 10, debug.level = 0, method = "glm.fit", 
    printit = TRUE, cvparts = NULL, seed = 29){
  # modified from the `gamclass` package to use gam::gam
    if (is.null(cvparts)) {
        set.seed(seed)
        cvparts <- sample(1:nfold, nrow(data), replace = TRUE)
    }
    folds <- unique(cvparts)
    khat <- hat <- numeric(nrow(data))
    scale.gam <- summary(gam::gam(formula, data = data, method = method,
                                  family = mgcv::betar(link = "logit", eps = 0.00001)))$scale
    for (i in folds) {
        trainrows <- cvparts != i
        testrows <- cvparts == i
        elev.gam <- gam::gam(formula, data = data[trainrows, ], 
                             method = method, 
                             family = mgcv::betar(link = "logit", eps = 0.00001))
        hat[testrows] <- predict(elev.gam, newdata = data[testrows,], select = T, type = "response")
        res <- residuals(elev.gam)
    }
    y <- eval(formula[[2]], envir = as.data.frame(data))
    res <- y - hat
    cvscale <- sum(res^2)/length(res)
    prntvec <- c(GAMscale = scale.gam, `CV-mse-GAM ` = cvscale)
    if (printit) 
        print(round(prntvec, 4))
    invisible(list(fitted = hat, resid = res, cvscale = cvscale, 
        scale.gam = scale.gam))
}
fittingFunc = function(g){
  t = sub_dat$zonation_pt
  gene_fit_p = c()
  gene_fit_vals = data.frame(row.names = 1:100)
  z = sub_dat@assays$SCT@data[g,]
    
  d = data.frame(z=z, t=t)
  tmp = suppressMessages(gam(z ~ ns(t, df = 3), data=d))
  
  # bins for model fitting
  bb = seq(min(t), max(t), length.out = 100)
  gene_fit_vals[,g] = suppressMessages(predict(tmp,
                                               newdata = data.frame(t = bb)))
  p = summary(tmp)$parametric.anova$`Pr(>F)`[1]
  gene_fit_p = c(gene_fit_p, p)
  names(gene_fit_p)[length(gene_fit_p)] = g
  
  return(list("fits" = gene_fit_vals, "pval" = gene_fit_p))
}
minmax_scale = function(x){
  return((x-min(x))/(max(x)-min(x)))
}
getTopTerms = function(godf, topt = 100, ncl = 5, nt = 2){
  topt = if(nrow(godf)<topt) nrow(godf) else topt
  if(nrow(godf)<ncl) return(godf)
  df = godf[1:topt,]
  genes = sapply(df$geneID, function(x) strsplit(x, "/"))
  resmat = matrix(0, length(genes), length(genes))
  for(i in 1:length(genes)){
    for(j in 1:length(genes)){
      resmat[i,j] = length(intersect(genes[[i]], genes[[j]]))/length(genes[[i]])
    }
  }
  cl = hclust(dist(resmat), method = "ward.D2")
  cl = cutree(cl, ncl)
  res_df = data.frame("Description" = df$Description, "qvalue" = df$qvalue, "geneID" = df$geneID, 
                      cl, stringsAsFactors = F)
  res_df = res_df[order(res_df$qvalue, decreasing = F),]
  topterms = unlist(tapply(res_df$Description, res_df$cl, function(x) x[1:nt]))
  res_df = res_df[res_df$Description %in% topterms,]
  
  return(res_df)
}
```

Load data (from all cells)

```{r}
allcells_css = readRDS(file = "data/processed/allcells_css.RDS")
```



# Endothelial cell clustering
Subset LSEC

```{r}
all_end_cells = allcells_css[,grepl("LSEC", allcells_css@meta.data$allcells_clusters)]
all_end_cells = suppressWarnings(SCTransform(all_end_cells, do.correct.umi = T, verbose = F, 
                                             vars.to.regress=c("unique_name","nCount_RNA"),
                                             variable.features.rv.th = 1, seed.use = 1,
                                             return.only.var.genes = F, 
                                             variable.features.n = NULL))
all_end_cells = RunPCA(all_end_cells, verbose = F)
all_end_cells = RunUMAP(all_end_cells, dims = 1:25, verbose = F)
DimPlot(all_end_cells, reduction = "umap", group.by = "Condition")
DimPlot(all_end_cells, reduction = "umap", group.by = "allcells_simp")
DimPlot(all_end_cells, reduction = "umap", group.by = "Donor")
DimPlot(all_end_cells, reduction = "umap", group.by = "Phase")
FeaturePlot(all_end_cells, reduction = "umap", features = c("MKI67", "ALB", "S100A8", "COLEC11",
                                                            "CLEC1B", "CLEC14A", "AQP1", "MGP", 
                                                            "LYVE1", "PROX1", "CD14", "PECAM1",
                                                            "VWF", "INMT", "PLVAP", "RBP7"))
```

Cluster and get markers

```{r}
all_end_cells = FindNeighbors(all_end_cells, reduction = "pca", dims = 1:25,
                              prune.SNN = 1/5, force.recalc = T, graph.name = "pca25")
all_end_cells = FindClusters(all_end_cells, algorithm = 2, verbose = F, graph.name = "pca25",
                             resolution = seq(0.1, 2, 0.1))
DimPlot(all_end_cells, reduction = "umap", group.by = "pca25_res.1", label = T)
DimPlot(all_end_cells, reduction = "umap", group.by = "Donor", label = F)
DimPlot(all_end_cells, reduction = "umap", group.by = "Condition", label = F)

all_end_cells = SetIdent(all_end_cells, value = "pca25_res.1")
mkend = FindAllMarkers(all_end_cells, logfc.threshold = 0.2, pseudocount.use = 0.1)
write.csv(mkend[mkend$p_val_adj<=0.05,], 
          file = "results/endothelial/markers_endo_subpop_all.csv", row.names = T, quote = F)

saveRDS(mkend, file = "./results/endothelial/clust_markers_endo.RDS")
```

Annotate the clusters

```{r}
annot = c("0" = "Periportal LSEC",
"1" = "LSEC (stress)",
"2" = "EC non-LSEC",
"3" = "Pericentral LSEC",
"4" = "Periportal LSEC",
"5" = "Pericentral LSEC",
"6" = "LSEC (remodelling)",
"7" = "LSEC (high MT 1)",
"8" = "LSEC (interferon)",
"9" = "B cell/Hepatocyte/EC doublets",
"10" = "LSEC (high MT 2)",
"11" = "Unknown_D3_emb",
"12" = "Unknown_D3_regen",
"13" = "LSEC (fenestr.)",
"14" = "Hepatocytes",
"15" = "Lymphatic EC",
"16" = "Cycling cells",
"17" = "Stellate cells")

clnames = data.frame("endo_annot" = all_end_cells@meta.data$pca25_res.1)
rownames(clnames) = colnames(all_end_cells)
clnames$endo_spec = plyr::revalue(clnames$endo_annot, annot)
clnames$endo_simp = as.character(clnames$endo_spec)
clnames$endo_simp[clnames$endo_annot %in% c(9, 11, 12, 14, 17)] = "Other/unknown"

all_end_cells = AddMetaData(all_end_cells, metadata = clnames)
DimPlot(all_end_cells, reduction = "umap", group.by = "endo_simp", label = F)

saveRDS(all_end_cells, file = "./results/endothelial/all_end_cells.RDS")
```

Redo all w/o contaminating cells (only ECs of any type)

```{r}
only_end_cells = all_end_cells[,!grepl("Other/unknown", all_end_cells@meta.data$endo_simp)]
only_end_cells = suppressWarnings(SCTransform(only_end_cells, do.correct.umi = T, verbose = F, 
                                             vars.to.regress=c("unique_name","nCount_RNA"),
                                             variable.features.rv.th = 1, seed.use = 1,
                                             return.only.var.genes = F, 
                                             variable.features.n = NULL))
only_end_cells = RunPCA(only_end_cells, verbose = F)
only_end_cells = RunUMAP(only_end_cells, dims = 1:25, verbose = F)

only_end_cells = FindNeighbors(only_end_cells, reduction = "pca", dims = 1:25,
                              prune.SNN = 1/5, force.recalc = T, graph.name = "pca25")
only_end_cells = FindClusters(only_end_cells, algorithm = 2, verbose = F, graph.name = "pca25",
                             resolution = seq(0.1, 2, 0.1))
DimPlot(only_end_cells, reduction = "umap", group.by = "pca25_res.0.9", label = T)
DimPlot(only_end_cells, reduction = "umap", group.by = "endo_simp", label = T)
DimPlot(only_end_cells, reduction = "umap", group.by = "Donor", label = F)
DimPlot(only_end_cells, reduction = "umap", group.by = "Condition", label = F)
```

Markers for filtered clusters

```{r}
only_end_cells = SetIdent(only_end_cells, value = "pca25_res.0.9")
mkend = FindAllMarkers(only_end_cells, logfc.threshold = 0.2, pseudocount.use = 0.1)
write.csv(mkend[mkend$p_val_adj<=0.05,], 
          file = "results/endothelial/markers_endo_subpop_only.csv", row.names = T, quote = F)
saveRDS(mkend, file = "./results/endothelial/clust_markers_endo_only.RDS")
```

Annotate the clusters for ECs only

```{r}
annot = c("0" = "Midzonal LSEC",
"1" = "Pericentral LSEC",
"2" = "LSEC (stress)",
"3" = "Periportal LSEC",
"4" = "EC non-LSEC",
"5" = "LSEC (interferon)",
"6" = "LSEC (remodelling)",
"7" = "LSEC (high MT 1)",
"8" = "LSEC (high MT 2)",
"9" = "LSEC (fenestr.)",
"10" = "Lymphatic EC",
"11" = "Cycling cells",
"12" = "Periportal LSEC")

clnames = data.frame("endoOnly_annot" = only_end_cells@meta.data$pca25_res.0.9)
rownames(clnames) = colnames(only_end_cells)
clnames$endo_spec = plyr::revalue(clnames$endoOnly_annot, annot)
clnames$endo_simp = as.character(clnames$endo_spec)

only_end_cells = AddMetaData(only_end_cells, metadata = clnames)
DimPlot(only_end_cells, reduction = "umap", group.by = "endo_simp", label = F)

saveRDS(only_end_cells, file = "./results/endothelial/only_end_cells.RDS")
```

Get condition markers for each cluster

```{r}
only_end_cells = SetIdent(only_end_cells, value = "Condition")
mk_endo_list = list()
for(ct in unique(only_end_cells$endo_simp)){
  mk_endo_list[[ct]] = FindAllMarkers(only_end_cells[,only_end_cells$endo_simp==ct], assay = "SCT",
                                      pseudocount.use = 0.1, logfc.threshold = 0.2)
  mk_endo_list[[ct]] = mk_endo_list[[ct]][mk_endo_list[[ct]]$p_val_adj<=0.05,]
}
saveRDS(mk_endo_list, file = "./results/endothelial/cond_markers_endo.RDS")
```

Plot abundance of clusters per condition

```{r, fig.width=4}
df_cnt = table(only_end_cells$endo_simp, only_end_cells$Condition)
df_cnt_perCond = reshape2::melt(apply(df_cnt, 2, function(x) round(x/sum(x)*100, 1)))
df_cnt_perCl = reshape2::melt(apply(df_cnt, 1, function(x) round(x/sum(x)*100, 1)))

mat_cnt_all = reshape2::dcast(data = df_cnt_perCond, formula = Var1 ~ Var2, value.var = "value")
rownames(mat_cnt_all) = mat_cnt_all$Var1
mat_cnt_all = mat_cnt_all[,-1]
ctord = hclust(dist(mat_cnt_all[,c(2,1,3)]))$order

heatp = pheatmap::pheatmap(mat_cnt_all[ctord,c(2,1,3)], cluster_cols = F, cluster_rows = F, 
                           treeheight_row = F, display_numbers = T, 
                           number_color = c("black","white")[as.integer(mat_cnt_all[ctord,c(2,1,3)]>25)+1],
                           color = colorRampPalette(brewer.pal(n = 9, name = "Blues"))(100),
                           fontsize_row = 8, fontsize_col = 8, angle_col = 0)

mat_cnt_all = reshape2::dcast(data = df_cnt_perCl, formula = Var1 ~ Var2, value.var = "value")
rownames(mat_cnt_all) = mat_cnt_all$Var1
mat_cnt_all = t(mat_cnt_all[,-1])
ctord = hclust(dist(mat_cnt_all[,c(2,1,3)]))$order

heatp = pheatmap::pheatmap(mat_cnt_all[ctord,c(2,1,3)], cluster_cols = F, cluster_rows = F, 
                           treeheight_row = F, display_numbers = T, 
                           number_color = c("black", "white")[as.integer(mat_cnt_all[ctord,c(2,1,3)]>50)+1],
                           color = colorRampPalette(brewer.pal(n = 9, name = "Blues"))(100),
                           fontsize_row = 8, fontsize_col = 8, angle_col = 0)

only_end_cells = SetIdent(only_end_cells, value = "endo_simp")
mkend_simp = FindAllMarkers(only_end_cells, logfc.threshold = 0.2, pseudocount.use = 0.1)
write.csv(mkend_simp[mkend_simp$p_val_adj<=0.05,], 
          file = "results/endothelial/markers_endo_subpop_simp.csv", row.names = T, quote = F)
```



# Endothelial zonation
Make different subsets to test

```{r}
lsec_list = list(lsec_end_cells = only_end_cells[,only_end_cells$pca25_res.0.9 %in% c(0, 1, 3, 12)])
```

Normalise, PCA, UMAP

```{r}
h_lsec_list = list()
for(n in names(lsec_list)){
  h_lsec_list[[n]] = lsec_list[[n]][,lsec_list[[n]]$Condition=="healthy"]
  h_lsec_list[[n]] = suppressWarnings(SCTransform(h_lsec_list[[n]], do.correct.umi = T, verbose = F, 
                                                  vars.to.regress=c("unique_name","nCount_RNA"),
                                                  variable.features.rv.th = 1, seed.use = 1,
                                                  return.only.var.genes = F, 
                                                  variable.features.n = NULL))
  h_lsec_list[[n]] = RunPCA(h_lsec_list[[n]], verbose = F)
  h_lsec_list[[n]] = RunUMAP(h_lsec_list[[n]], dims = 1:20, verbose = F)
}
```

Get DPT

```{r}
dpt_l = list()
for(n in names(h_lsec_list)){
  set.seed(1)
  dm = DiffusionMap(h_lsec_list[[n]]@reductions$pca@cell.embeddings[,1:10], 
                    rotate = T, n_eigs = 5)
  dpt = DPT(dm)
  plot(dpt, main = n)
  dpt_l[[n]] = dpt
}
```

Plot DPT as violin

```{r}
for(n in names(h_lsec_list)){
  plot_df = data.frame(row.names = rownames(dpt_l[[n]]@branch), 
                       "cl" = h_lsec_list[[n]]$endo_simp,
                       "dpt" = getDPT(dpt_l[[n]]))
  
  plt = ggplot(plot_df, aes(y = cl, x = dpt, fill = cl))+
    geom_boxplot()+
    labs(title = n)+
    theme_bw()+
    theme(axis.text.x = element_text(size = 7.5))
  print(plt)
}
```

We're using subset 2 - only bona fide LSEC

```{r}
# filter some outlier cells and reprocess
cond = dpt_l$lsec_end_cells@dm$DC1<(0.1)
sub_2 = h_lsec_list$lsec_end_cells[,cond]
sub_2 = suppressWarnings(SCTransform(sub_2, do.correct.umi = T, verbose = F, 
                                     vars.to.regress=c("unique_name","nCount_RNA"),
                                     variable.features.rv.th = 1, seed.use = 1,
                                     return.only.var.genes = F, variable.features.n = NULL))
sub_2 = RunPCA(sub_2, verbose = F)
sub_2 = RunUMAP(sub_2, dims = 1:20, verbose = F)
DimPlot(sub_2, reduction = "umap", group.by = "endo_simp")

set.seed(1)
dm = DiffusionMap(sub_2@reductions$pca@cell.embeddings[,1:10], 
                  rotate = T, n_eigs = 2)
dpt = DPT(dm)
plot(dpt)
```

Plot pseudotime as boxplot

```{r}
plot_df = data.frame(row.names = rownames(dpt@branch), 
                     "cl" = sub_2$endo_simp,
                     "dpt" = getDPT(dpt))

plt = ggplot(plot_df, aes(y = cl, x = rank(dpt), fill = cl))+
  geom_boxplot()+
  theme_bw()+
  theme(axis.text.x = element_text(size = 7.5))
print(plt)
```

Find genes varying along the pseudotime

```{r}
df_dc = cbind(sub_2@meta.data, 
              data.frame("DC1" = dpt@dm$DC1, "DC2" = dpt@dm$DC2, 
                         "DPT" = scales::rescale(rank(getDPT(dpt)), c(0.00001, 0.99999))))

# will only use variable genes
hvg = sub_2@assays$SCT@var.features

# Fit GAM for each gene using pseudotime as independent variable.
t = df_dc$DPT
gene_fit_p = c()
gene_fit_vals = data.frame(row.names = 1:100)
for(i in 1:length(hvg)){
  g = hvg[i]
  z = sub_2@assays$SCT@data[g,]
  
  d = data.frame(z=z, t=t)
  tmp = suppressMessages(gam(z ~ ns(t, df = 3), data=d))
  
  # bins for model fitting
  bb = seq(min(t), max(t), length.out = 100)
  gene_fit_vals[,g] = suppressMessages(predict(tmp,
                                               newdata = data.frame(t = bb)))
  p = summary(tmp)$parametric.anova$`Pr(>F)`[1]
  gene_fit_p = c(gene_fit_p, p)
  names(gene_fit_p)[length(gene_fit_p)] = g
}

gene_fit_p = fdrtool::fdrtool(gene_fit_p, statistic="pvalue", plot=F, 
                              verbose=F, cutoff.method="pct0", pct0=0.9)$qval
```

Now use the top genes to learn the pseudozonation coordinates and project the embolised and regenerating data

```{r}
sig_genes = gene_fit_p[gene_fit_p<=0.05]
top_sig_genes = names(sig_genes[order(sig_genes, decreasing = F)][1:1000])
top_sig_genes = top_sig_genes[top_sig_genes %in% rownames(only_end_cells@assays$SCT@data)]

data_gam_h = cbind(data.frame("DPT" = df_dc$DPT),
                   Matrix::t(sub_2@assays$SCT@data[top_sig_genes,]))

gam_healthy = gam::gam(DPT ~ ., data = data_gam_h, 
                       family = mgcv::betar(link = "logit", eps = 0.00001))

all_pred = predict(gam_healthy,
                   Matrix::t(only_end_cells@assays$SCT@data[top_sig_genes,]), type= "response")

goodcl = only_end_cells$pca25_res.0.9 %in% sub_2$pca25_res.0.9
all_pred_sub = all_pred[goodcl & only_end_cells$Condition!="healthy" | names(all_pred) %in% colnames(sub_2)]

traj_list = list("healthy" = data_gam_h$DPT, 
                 "embolised" = all_pred[goodcl & only_end_cells$Condition!="embolised"], 
                 "regenerating" = all_pred[goodcl & only_end_cells$Condition!="regenerating"])

only_end_cells = AddMetaData(only_end_cells, 
                             data.frame("zonation_pt" = all_pred, 
                                        "goodcl" = colnames(only_end_cells) %in% names(all_pred_sub)))
only_end_cells@meta.data[rownames(data_gam_h),"zonation_pt"] = data_gam_h$DPT

plot_df = only_end_cells@meta.data[names(all_pred_sub),]
plot_df$bins100 = cut(plot_df$zonation_pt, 10)
plot_df$Condition = factor(plot_df$Condition, levels = c("healthy", "embolised", "regenerating"))
ggplot(plot_df, aes(x = bins100, fill = endo_simp))+
     facet_wrap(~Condition)+
     geom_bar()+
     labs(x = "zonation (binned)", y = "Number of cells")+
     scale_y_continuous(expand = c(0,0))+
     coord_flip()+
     guides(fill = guide_legend(title.position = "top"))+
  theme_classic()+
     theme(axis.text.y = element_blank(),
           strip.background = element_rect(fill = colcond),
           legend.title.align = 0,
           legend.position = "bottom")
VlnPlot(only_end_cells[,names(all_pred_sub)], features = "nCount_RNA", 
        group.by = "endo_simp", split.by = "Condition")
ggplot(plot_df, aes(x = zonation_pt, y = endo_simp, fill = endo_simp))+
     facet_wrap(~Condition)+
     geom_violin()+
     labs(x = "zonation", y = "clusters")+
     scale_x_continuous(expand = c(0,0))+
     guides(fill = guide_legend(title.position = "top"))+
     theme_bw()+
     theme(axis.text.y = element_blank(),
           legend.title.align = 0,
           legend.position = "bottom")
```



```{r}
plot_df = only_end_cells@meta.data
plot_df$pt = all_pred[colnames(only_end_cells)]
plot_df$bins100 = cut(plot_df$pt, 10)
plot_df$Condition = factor(plot_df$Condition, levels = c("healthy", "embolised", "regenerating"))

ggplot(plot_df, aes(x = pt, y = endo_simp, fill = endo_simp))+
     facet_wrap(~Condition)+
     geom_violin()+
     labs(x = "zonation", y = "clusters")+
     scale_x_continuous(expand = c(0,0))+
     guides(fill = guide_legend(title.position = "top", reverse = T))+
     theme_bw()+
     theme(axis.text.y = element_blank(),
           legend.title.align = 0,
           legend.position = "right")
```

Do cross validation on the healthy trajectory

```{r}
sig_genes = gene_fit_p[gene_fit_p<=0.05]
top_sig_genes = names(sig_genes[order(sig_genes, decreasing = F)][1:1000]) 

data_gam_h = cbind(data.frame("DPT" = traj_list$healthy),
                   Matrix::t(sub_2@assays$SCT@data[top_sig_genes,]))
colnames(data_gam_h) = gsub("-", "_", colnames(data_gam_h), fixed = T)
colnames(data_gam_h) = gsub(".", "_", colnames(data_gam_h), fixed = T)

cv_gam = CVgam(data = data_gam_h, formula = DPT ~ ., nfold = 10, seed = 1, method = "glm.fit")

pdf("results/endothelial/end_original_fitted_pseudotime.pdf", useDingbats = F, 
    width = 6, height = 5)
plot(data_gam_h$DPT, cv_gam$fitted, xlab = "Healthy pseudotime",ylab = "Predicted Healthy",
     main = paste0("Healthy original vs fitted pseudotime\nMSE: ", round(cv_gam$cvscale, 6)),
     pch = 19, cex = 0.5)
abline(0,1, col = "blue", lwd = 3)
dev.off()

plot_df = data.frame("original" = data_gam_h$DPT, "predicted" = cv_gam$fitted)
plot_df$cl = sub_2@meta.data$endo_simp
ggplot(plot_df, aes(x = original, y = predicted, colour = cl))+geom_point()
```

Find varying genes - using all cells along the ranked pseudotime

```{r}
## get varying genes for each condition
numCores = 12 #larger values resulted in problems
start_time <- Sys.time()
cond_fit_list = list()

only_end_cells_bf = only_end_cells[,only_end_cells$pca25_res.0.9 %in% sub_2$pca25_res.0.9]
only_end_cells_split = SplitObject(only_end_cells_bf, split.by = "Condition")

for(cond in names(only_end_cells_split)){
  print(cond)
  
  sub_dat = only_end_cells_split[[cond]]
  # Fit GAM for each gene using pseudotime as independent variable.
  cond_fit_list[[cond]] = mclapply(hvg, fittingFunc, mc.cores = numCores)
}
end_fits_qval = list()
for(cond in names(cond_fit_list)){
  fits_df = Reduce(cbind, lapply(cond_fit_list[[cond]], function(x) x$fits))
  
  pval_list = unlist(lapply(cond_fit_list[[cond]], function(x) x$pval))
  names(pval_list) = hvg
  
  pval_df = data.frame(pval_list)
  
  end_fits_qval[[cond]] = list("fits" = fits_df, "pval" = pval_df,
                               "fdr" = apply(pval_df, 2, function(x) p.adjust(x, method = "fdr")))
}
end_time <- Sys.time()
end_time - start_time

saveRDS(end_fits_qval, file = "results/endothelial/end_fits_qval.RDS")
```

Correlate genes between conditions

```{r}
g = "LYVE1"
plot(end_fits_qval$healthy$fits[,g])
points(end_fits_qval$embolised$fits[,g], col = "red")
points(end_fits_qval$regenerating$fits[,g], col = "blue")

all_var_genes = (end_fits_qval$healthy$fdr<=0.05 & 
  apply(end_fits_qval$healthy$fits, 2, function(x) diff(range(x))>=0.05)) | 
  (end_fits_qval$embolised$fdr<=0.05 & 
  apply(end_fits_qval$embolised$fits, 2, function(x) diff(range(x))>=0.05)) | 
  (end_fits_qval$regenerating$fdr<=0.05 & 
  apply(end_fits_qval$regenerating$fits, 2, function(x) diff(range(x))>=0.05))

end_cor_he = sapply(rownames(all_var_genes)[all_var_genes], 
                    function(g) cor(end_fits_qval$healthy$fits[,g],end_fits_qval$embolised$fits[,g], 
                                    method = "sp"), simplify = T)
end_cor_hr = sapply(rownames(all_var_genes)[all_var_genes], 
                    function(g) cor(end_fits_qval$healthy$fits[,g],end_fits_qval$regenerating$fits[,g], 
                                    method = "sp"), simplify = T)
end_cor_er = sapply(rownames(all_var_genes)[all_var_genes], 
                    function(g) cor(end_fits_qval$embolised$fits[,g],end_fits_qval$regenerating$fits[,g], 
                                    method = "sp"), simplify = T)

cor_g_df = data.frame("gene" = names(end_cor_he), 
                      "HvE" = end_cor_he, "HvR" = end_cor_hr, "EvR" = end_cor_er)
write.csv(cor_g_df, file = "results/endothelial/correlations_zonation_endothelial.csv", 
          row.names = T, quote = F)
```

GO Term enrichment

```{r}
got_cor = list()
eg_all = clusterProfiler::bitr(colnames(end_fits_qval$healthy$fits), fromType="SYMBOL", 
                             toType="ENTREZID", OrgDb="org.Hs.eg.db")

eg = clusterProfiler::bitr(names(end_cor_he)[end_cor_he<0.3], fromType="SYMBOL", 
                           toType="ENTREZID", OrgDb="org.Hs.eg.db")
got = clusterProfiler::enrichGO(gene = eg$ENTREZID, universe = eg_all$ENTREZID, 
                                OrgDb = org.Hs.eg.db, ont = "BP", pvalueCutoff  = 0.01,
                                qvalueCutoff  = 0.05, 
                                pAdjustMethod = "BH", readable = T)
got_cor[["notcor_he"]] = getTopTerms(got@result[got@result$p.adjust<=0.05,], ncl = 4, nt = 2, topt = 1000)

eg = clusterProfiler::bitr(names(end_cor_he)[end_cor_he>=0.3], fromType="SYMBOL", 
                           toType="ENTREZID", OrgDb="org.Hs.eg.db")
got = clusterProfiler::enrichGO(gene = eg$ENTREZID, universe = eg_all$ENTREZID, 
                                OrgDb = org.Hs.eg.db, ont = "BP", pvalueCutoff  = 0.01,
                                qvalueCutoff  = 0.05, 
                                pAdjustMethod = "BH", readable = T)
got_cor[["cor_he"]] = getTopTerms(got@result[got@result$p.adjust<=0.05,], ncl = 4, nt = 2, topt = 1000)

eg = clusterProfiler::bitr(names(end_cor_hr)[end_cor_hr<0.3], fromType="SYMBOL", 
                           toType="ENTREZID", OrgDb="org.Hs.eg.db")
got = clusterProfiler::enrichGO(gene = eg$ENTREZID, universe = eg_all$ENTREZID, 
                                OrgDb = org.Hs.eg.db, ont = "BP", pvalueCutoff  = 0.01,
                                qvalueCutoff  = 0.05, 
                                pAdjustMethod = "BH", readable = T)
got_cor[["notcor_hr"]] = getTopTerms(got@result[got@result$p.adjust<=0.05,], ncl = 4, nt = 2, topt = 1000)

eg = clusterProfiler::bitr(names(end_cor_hr)[end_cor_hr>=0.3], fromType="SYMBOL", 
                           toType="ENTREZID", OrgDb="org.Hs.eg.db")
got = clusterProfiler::enrichGO(gene = eg$ENTREZID, universe = eg_all$ENTREZID, 
                                OrgDb = org.Hs.eg.db, ont = "BP", pvalueCutoff  = 0.01,
                                qvalueCutoff  = 0.05, 
                                pAdjustMethod = "BH", readable = T)
got_cor[["cor_hr"]] = getTopTerms(got@result[got@result$p.adjust<=0.05,], ncl = 4, nt = 2, topt = 1000)

saveRDS(got_cor, file = "results/endothelial/GOTerms_correlations.RDS")
```

Plot top genes in correlation

```{r}
topgenes = unique(c(names(end_cor_he[order(end_cor_he, decreasing = T)])[1:30],
             names(end_cor_hr[order(end_cor_hr, decreasing = T)])[1:30]))
bottomgenes = unique(c(names(end_cor_he[order(end_cor_he, decreasing = F)])[1:30],
             names(end_cor_hr[order(end_cor_hr, decreasing = F)])[1:30]))
usegenes = c(topgenes, bottomgenes)

bins_list = list()
for(n in names(only_end_cells_split)){
  plot_df = cbind(data.frame("pt" = rep(1:10, each = 10)),end_fits_qval[[n]]$fits[,usegenes])
  plot_df$bins10 = cut(plot_df$pt, 10)
  bins_list[[n]] = sapply(usegenes, function(x) scale(tapply(plot_df[,x], 
                                                             plot_df$bins10, mean))[10:1])
}
bins_mean = Reduce(rbind, bins_list)

hct = hclust(dist(t(bins_list$healthy[,topgenes])), method = "ward.D2")
ord_bot_h = apply(bins_list$healthy[,bottomgenes], 2, which.max)
ord_bot_e = apply(bins_list$embolised[,bottomgenes], 2, which.max)
ord_bot_r = apply(bins_list$regenerating[,bottomgenes], 2, which.max)
ord_df = data.frame(ord_bot_h, ord_bot_e, ord_bot_r)
ord_bot = rownames(ord_df)[with(ord_df, order(ord_bot_h, ord_bot_e, ord_bot_r, decreasing = T))]
ord = c(topgenes[hct$order], ord_bot)

pheatmap::pheatmap(bins_mean[,ord], show_rownames = F, fontsize_col = 6.5, border_color = NA,
                   gaps_row = c(10,20), gaps_col = c(length(topgenes)),
                   clustering_method = "ward.D2", cluster_rows = F, cluster_cols = F)
```

Save Seurat with predictions

```{r}
saveRDS(only_end_cells, file = "results/endothelial/only_end_cells_zon.RDS")
```











